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Literature DB >> 23256865

Engineered rRNA enhances the efficiency of selenocysteine incorporation during translation.

Ross Thyer¹, Aleksandra Filipovska, Oliver Rackham.

Abstract

We developed a new genetic selection approach to screen for mutations that can alter the efficiency of selenocysteine incorporation. We identified mutations in 16S rRNA that increase or decrease the efficiency of selenocysteine incorporation in Escherichia coli without influencing the efficiency or fidelity of canonical translation. Engineered ribosomes with improved selenocysteine incorporation provide valuable tools for synthetic biology and biotechnology.

Entities: Chemical Disease Species

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Year: 2012 PMID： 23256865 DOI： 10.1021/ja3069177

Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419

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Cited

18 in total

1. Directed evolution of genetic parts and circuits by compartmentalized partnered replication.

Authors: Jared W Ellefson; Adam J Meyer; Randall A Hughes; Joe R Cannon; Jennifer S Brodbelt; Andrew D Ellington
Journal: Nat Biotechnol Date: 2013-11-03 Impact factor: 54.908

2. Selenocysteine Insertion at a Predefined UAG Codon in a Release Factor 1 (RF1)-depleted Escherichia coli Host Strain Bypasses Species Barriers in Recombinant Selenoprotein Translation.

Authors: Qing Cheng; Elias S J Arnér
Journal: J Biol Chem Date: 2017-02-13 Impact factor: 5.157

3. Utilizing Selenocysteine for Expressed Protein Ligation and Bioconjugations.

Authors: Jun Liu; Qingqing Chen; Sharon Rozovsky
Journal: J Am Chem Soc Date: 2017-02-27 Impact factor: 15.419

Review 4. Chemically programmed antibodies.

Authors: Christoph Rader
Journal: Trends Biotechnol Date: 2014-03-11 Impact factor: 19.536

Review 5. Rewriting the Genetic Code.

Authors: Takahito Mukai; Marc J Lajoie; Markus Englert; Dieter Söll
Journal: Annu Rev Microbiol Date: 2017-07-11 Impact factor: 15.500

6. Site-Specific Incorporation of Selenocysteine Using an Expanded Genetic Code and Palladium-Mediated Chemical Deprotection.

Authors: Jun Liu; Feng Zheng; Rujin Cheng; Shanshan Li; Sharon Rozovsky; Qian Wang; Lei Wang
Journal: J Am Chem Soc Date: 2018-07-09 Impact factor: 15.419

Engineered rRNA enhances the efficiency of selenocysteine incorporation during translation.

1. Directed evolution of genetic parts and circuits by compartmentalized partnered replication.

2. Selenocysteine Insertion at a Predefined UAG Codon in a Release Factor 1 (RF1)-depleted Escherichia coli Host Strain Bypasses Species Barriers in Recombinant Selenoprotein Translation.

3. Utilizing Selenocysteine for Expressed Protein Ligation and Bioconjugations.

Review 4. Chemically programmed antibodies.

Review 5. Rewriting the Genetic Code.

6. Site-Specific Incorporation of Selenocysteine Using an Expanded Genetic Code and Palladium-Mediated Chemical Deprotection.

Review 7. Repurposing ribosomes for synthetic biology.

Review 8. Synthesis and semisynthesis of selenopeptides and selenoproteins.

9. Generation of Recombinant Mammalian Selenoproteins through Genetic Code Expansion with Photocaged Selenocysteine.

Review 10. Chemical Biology Approaches to Interrogate the Selenoproteome.