| Literature DB >> 23256573 |
Dmitry S Bilan1, Luke Pase, Linda Joosen, Andrey Yu Gorokhovatsky, Yulia G Ermakova, Theodorus W J Gadella, Clemens Grabher, Carsten Schultz, Sergey Lukyanov, Vsevolod V Belousov.
Abstract
High-performance sensors for reactive oxygen species are instrumental to monitor dynamic events in cells and organisms. Here, we present HyPer-3, a genetically encoded fluorescent indicator for intracellular H2O2 exhibiting improved performance with respect to response time and speed. HyPer-3 has an expanded dynamic range compared to HyPer and significantly faster oxidation/reduction dynamics compared to HyPer-2. We demonstrate this performance by in vivo imaging of tissue-scale H2O2 gradients in zebrafish larvae. Moreover, HyPer-3 was successfully employed for single-wavelength fluorescent lifetime imaging of H2O2 levels both in vitro and in vivo.Entities:
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Year: 2013 PMID： 23256573 DOI： 10.1021/cb300625g
Source DB: PubMed Journal: ACS Chem Biol ISSN： 1554-8929 Impact factor: 5.100