Literature DB >> 23255201

Synthesis of a North-methanocarba-thymidine (N-MCT) analog.

Andrew Thompson1, Victor E Marquez.   

Abstract

A detailed protocol for the synthesis of North-methanocarba-thymidine (n class="Chemical">N-MCT), a potent antiviral nucleoside with a restricted bicyclo[3.1.0]hexane pseudosugar conformation, is presented. The process is described in two parts. The first basic protocol deals with the synthesis of the carbobicyclic pseudosugar precursor that can be utilized in the syntheses of other bicyclo[3.1.0]hexane nucleosides with natural and non-natural nucleobases. The second basic protocol describes the specific construction of the thymine base in a linear fashion from the carbobicyclic intermediate.
© 2012 by John Wiley & Sons, Inc.

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Year:  2012        PMID: 23255201      PMCID: PMC7477758          DOI: 10.1002/0471142700.nc0129s51

Source DB:  PubMed          Journal:  Curr Protoc Nucleic Acid Chem        ISSN: 1934-9270


  6 in total

1.  The conformationally constrained N-methanocarba-dT analogue adopts an unexpected C4'-exo sugar pucker in the structure of a DNA hairpin.

Authors:  Pradeep S Pallan; Victor E Marquez; Martin Egli
Journal:  Biochemistry       Date:  2012-03-20       Impact factor: 3.162

2.  Changes in DNA bending induced by restricting nucleotide ring pucker studied by weak alignment NMR spectroscopy.

Authors:  Zhengrong Wu; Melissa Maderia; Joseph J Barchi; Victor E Marquez; Ad Bax
Journal:  Proc Natl Acad Sci U S A       Date:  2004-12-23       Impact factor: 11.205

3.  Effect of north bicyclo[3.1.0]hexane 2'-deoxy-pseudosugars on RNA interference: a novel class of siRNA modification.

Authors:  Montserrat Terrazas; Sandra M Ocampo; José Carlos Perales; Victor E Marquez; Ramon Eritja
Journal:  Chembiochem       Date:  2011-03-30       Impact factor: 3.164

4.  Nucleosides with a twist. Can fixed forms of sugar ring pucker influence biological activity in nucleosides and oligonucleotides?

Authors:  V E Marquez; M A Siddiqui; A Ezzitouni; P Russ; J Wang; R W Wagner; M D Matteucci
Journal:  J Med Chem       Date:  1996-09-13       Impact factor: 7.446

Review 5.  The history of N-methanocarbathymidine: the investigation of a conformational concept leads to the discovery of a potent and selective nucleoside antiviral agent.

Authors:  Victor E Marquez; Stephen H Hughes; Shizuko Sei; Riad Agbaria
Journal:  Antiviral Res       Date:  2006-05-06       Impact factor: 10.103

6.  Biophysical studies of DNA modified with conformationally constrained nucleotides: comparison of 2'-exo (north) and 3'-exo (south) 'locked' templates.

Authors:  Melissa Maderia; Shilpa Shenoy; Que N Van; Victor E Marquez; Joseph J Barchi
Journal:  Nucleic Acids Res       Date:  2007-03-06       Impact factor: 16.971

  6 in total

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