Fluorescent proteins as visible in vivo sensors.

Fluorescent proteins have enabled a whole new technology of visible in vivo genetic sensors. Fluorescent proteins have revolutionized biology by enabling what was formerly invisible to be seen clearly. These proteins have allowed us to visualize, in real time, important aspects of cancer in living animals, including tumor cell mobility, invasion, metastasis, and angiogenesis. These multicolored proteins have allowed the color coding of cancer cells growing in vivo and enabled the distinction of host from tumor with single-cell resolution. Whole-body imaging with fluorescent proteins has been shown to be a powerful technology to noninvasively follow the dynamics of metastatic cancer. Whole-body imaging of cancer cells expressing fluorescent proteins has enabled the facile determination of efficacy of candidate antitumor and antimetastatic agents in mouse models. The use of fluorescent proteins to differentially label cancer cells in the nucleus and cytoplasm and high-powered imaging technology have enabled the visualization of the nuclear-cytoplasmic dynamics of cancer cells in vivo, including noninvasive techniques. Fluorescent proteins thus enable both macro- and microimaging technology and thereby provide the basis for the new field of in vivo cell biology.