AIMS AND BACKGROUND: Myeloid differentiation primary response gene 88 (MYD88) is a protein involved in hematopoietic differentiation and innate immunity. Recent studies revealed MYD88 mutation in hematological malignancies and MYD88 overexpression in some solid cancers. The aim of this study was to see whether alterations of MYD88 protein expression and somatic mutation of MYD88 gene are features of common solid cancers. METHODS: We analyzed MYD88 mutation in 45 gastric, 45 colorectal, 45 breast, 45 hepatocellular, 45 prostate and 45 lung carcinomas by single-strand conformation polymorphism (SSCP). We also analyzed MYD88 protein expression in 60 gastric, 60 coloretal and 107 prostate carcinomas by immunohistochemistry. RESULTS: In the immunohistochemistry results, MYD88 protein was highly expressed in gastric (75%), colorectal (80%) and prostate (83%) cancers. However, MYD88 expression was significantly different among normal tissues (gastric: 58%, colon: 100%, prostate: 86%). MYD88 expression was significantly increased in gastric cancer cells compared with normal cells, whereas it was decreased in colorectal cancer cells compared with normal cells. There were no somatic mutations of the MYD88 gene in gastric, colorectal, breast, hepatocellular, prostate and lung carcinomas. CONCLUSIONS: Our data indicate that MYD88 overexpression might be a feature of many solid cancers, but MYD88 expression in normal cells differs depending on the organs. The data suggest that a gain of MYD88 expression in gastric cancers might play a role in cancer pathogenesis by activating oncogenic functions of MYD88.
AIMS AND BACKGROUND: Myeloid differentiation primary response gene 88 (MYD88) is a protein involved in hematopoietic differentiation and innate immunity. Recent studies revealed MYD88 mutation in hematological malignancies and MYD88 overexpression in some solid cancers. The aim of this study was to see whether alterations of MYD88 protein expression and somatic mutation of MYD88 gene are features of common solid cancers. METHODS: We analyzed MYD88 mutation in 45 gastric, 45 colorectal, 45 breast, 45 hepatocellular, 45 prostate and 45 lung carcinomas by single-strand conformation polymorphism (SSCP). We also analyzed MYD88 protein expression in 60 gastric, 60 coloretal and 107 prostate carcinomas by immunohistochemistry. RESULTS: In the immunohistochemistry results, MYD88 protein was highly expressed in gastric (75%), colorectal (80%) and prostate (83%) cancers. However, MYD88 expression was significantly different among normal tissues (gastric: 58%, colon: 100%, prostate: 86%). MYD88 expression was significantly increased in gastric cancer cells compared with normal cells, whereas it was decreased in colorectal cancer cells compared with normal cells. There were no somatic mutations of the MYD88 gene in gastric, colorectal, breast, hepatocellular, prostate and lung carcinomas. CONCLUSIONS: Our data indicate that MYD88 overexpression might be a feature of many solid cancers, but MYD88 expression in normal cells differs depending on the organs. The data suggest that a gain of MYD88 expression in gastric cancers might play a role in cancer pathogenesis by activating oncogenic functions of MYD88.
Authors: Christophe Cataisson; Rosalba Salcedo; Aleksandra M Michalowski; Mary Klosterman; Shruti Naik; Luowei Li; Michelle J Pan; Amalia Sweet; Jin-Qiu Chen; Laurie G Kostecka; Megan Karwan; Loretta Smith; Ren-Ming Dai; C Andrew Stewart; Lyudmila Lyakh; Wang-Ting Hsieh; Asra Khan; Howard Yang; Maxwell Lee; Giorgio Trinchieri; Stuart H Yuspa Journal: Mol Cancer Res Date: 2019-06-04 Impact factor: 5.852
Authors: Kathryn E Royse; Liang Chen; David H Berger; Michael M Ittmann; Hashem B El-Serag; Courtney J Balentine; David Y Graham; Peter A Richardson; Rolando E Rumbaut; Xiaoyun Shen; Donna L White; Li Jiao Journal: Int J Mol Epidemiol Genet Date: 2017-04-15
Authors: A C West; K Tang; H Tye; L Yu; N Deng; M Najdovska; S J Lin; J J Balic; E Okochi-Takada; P McGuirk; B Keogh; W McCormack; P S Bhathal; M Reilly; M Oshima; T Ushijima; P Tan; B J Jenkins Journal: Oncogene Date: 2017-05-08 Impact factor: 9.867
Authors: Lucas Daniel Trucco; Emiliano Roselli; Paula Araya; Nicolás Gonzalo Nuñez; Hebe Agustina Mena; José Luis Bocco; Soledad Negrotto; Mariana Maccioni Journal: PLoS One Date: 2017-06-29 Impact factor: 3.240