Literature DB >> 2323549

Isolation and characterization of a type II restriction endonuclease from Streptococcus thermophilus.

D K Solaiman1, G A Somkuti.   

Abstract

A type II restriction endonuclease Sth134I was isolated from Streptococcus thermophilus strain 134. The enzyme is an isoschizomer of HpaII. The restriction endonuclease is most active at Mg(II) greater than or equal to 5 mM; in the pH range of 7.5-8; temperature of 50 degrees-55 degrees C; and (KCl) or (NaCl) below 100 mM. Double digestion and ligation experiments showed that Sth134I apparently recognized and cleaves DNA at the sequence C1CGG to produce two-base, 5'-protruding ends.

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Year:  1990        PMID： 2323549     DOI： 10.1016/0378-1097(90)90006-c

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  8 in total

Review 1.  Restriction enzymes and their isoschizomers.

Authors:  R J Roberts; D Macelis
Journal:  Nucleic Acids Res       Date:  1991-04-25       Impact factor: 16.971

2.  Purification, properties, and sequence specificity of SslI, a new type II restriction endonuclease from Streptococcus salivarius subsp. thermophilus.

Authors:  L Benbadis; J R Garel; D L Hartley
Journal:  Appl Environ Microbiol       Date:  1991-12       Impact factor: 4.792

Review 3.  Bacteriophage resistance in Lactococcus.

Authors:  P K Dinsmore; T R Klaenhammer
Journal:  Mol Biotechnol       Date:  1995-12       Impact factor: 2.695

4.  Expression of antisense RNA targeted against Streptococcus thermophilus bacteriophages.

Authors:  Joseph M Sturino; Todd R Klaenhammer
Journal:  Appl Environ Microbiol       Date:  2002-02       Impact factor: 4.792

Review 5.  Biotechnology of lactic acid bacteria with special reference to bacteriophage resistance.

Authors:  C Daly; G F Fitzgerald; R Davis
Journal:  Antonie Van Leeuwenhoek       Date:  1996-10       Impact factor: 2.271

6.  Characterization of a novel type II restriction-modification system, Sth368I, encoded by the integrative element ICESt1 of Streptococcus thermophilus CNRZ368.

Authors:  V Burrus; C Bontemps; B Decaris; G Guédon
Journal:  Appl Environ Microbiol       Date:  2001-04       Impact factor: 4.792

7.  Expression of a Lactococcus lactis Phage Resistance Mechanism by Streptococcus thermophilus.

Authors:  S Moineau; S A Walker; B J Holler; E R Vedamuthu; P A Vandenbergh
Journal:  Appl Environ Microbiol       Date:  1995-07       Impact factor: 4.792

8.  Restriction/modification in Streptococcus thermophilus: isolation and characterization of a type II restriction endonuclease Sth455I.

Authors:  C Guimont; P Henry; G Linden
Journal:  Appl Microbiol Biotechnol       Date:  1993-05       Impact factor: 4.813
  8 in total

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