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Literature DB >> 2323545

Simplified procedure for purification of Bordetella bronchiseptica dermonecrotic toxin.

Abstract

Bordetella bronchiseptica dermonecrotic toxin was purified by a simplified method. The method consisted of SP Toyopearl 650M chromatography and high performance liquid chromatography on a TSK gel G3000SW column. 47.5% of the activity of the crude cell extract was recovered. The purified toxin behaved as a homogeneous protein in sodium dodecyl sulfate polyacrylamide gel electrophoresis, high performance liquid chromatography, and agar gel double diffusion tests.

Entities: Chemical Disease

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Year: 1990 PMID： 2323545 DOI： 10.1016/0378-1097(90)90255-o

Source DB: PubMed Journal: FEMS Microbiol Lett ISSN： 0378-1097 Impact factor: 2.742

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Cited

12 in total

1. In vivo modifications of small GTPase Rac and Cdc42 by Bordetella dermonecrotic toxin.

Authors: Minako Masuda; Masayoshi Minami; Hiroaki Shime; Takeshi Matsuzawa; Yasuhiko Horiguchi
Journal: Infect Immun Date: 2002-02 Impact factor: 3.441

2. Bordetella bronchiseptica dermonecrotizing toxin induces reorganization of actin stress fibers through deamidation of Gln-63 of the GTP-binding protein Rho.

Authors: Y Horiguchi; N Inoue; M Masuda; T Kashimoto; J Katahira; N Sugimoto; M Matsuda
Journal: Proc Natl Acad Sci U S A Date: 1997-10-14 Impact factor: 11.205

3. Effects of Bordetella bronchiseptica dermonecrotic toxin on the structure and function of osteoblastic clone MC3T3-e1 cells.

Authors: Y Horiguchi; T Nakai; K Kume
Journal: Infect Immun Date: 1991-03 Impact factor: 3.441

Simplified procedure for purification of Bordetella bronchiseptica dermonecrotic toxin.

1. In vivo modifications of small GTPase Rac and Cdc42 by Bordetella dermonecrotic toxin.

2. Bordetella bronchiseptica dermonecrotizing toxin induces reorganization of actin stress fibers through deamidation of Gln-63 of the GTP-binding protein Rho.

3. Effects of Bordetella bronchiseptica dermonecrotic toxin on the structure and function of osteoblastic clone MC3T3-e1 cells.

4. Cloning, expression, and molecular characterization of the dermonecrotic toxin gene of Bordetella spp.

5. Stimulation of DNA synthesis in osteoblast-like MC3T3-E1 cells by Bordetella bronchiseptica dermonecrotic toxin.

6. Activation of rho through a cross-link with polyamines catalyzed by Bordetella dermonecrotizing toxin.

7. Identification of functional domains of Bordetella dermonecrotizing toxin.

8. Association of Bordetella dermonecrotic toxin with the extracellular matrix.

9. Characterization of the dermonecrotic toxin in members of the genus Bordetella.

10. Identification of a receptor-binding domain of Bordetella dermonecrotic toxin.