Literature DB >> 2323185

Purification of C8 and C9 from rat serum.

J Jones1, I Laffafian, B P Morgan.   

Abstract

A procedure based on modifications of published methods for human proteins for the isolation of rat C8 and C9 from one batch of serum is described. The procedure allows the rapid, large-scale isolation of pure and haemolytically active proteins. Rat C9 had a slightly higher molecular weight than human C9 on SDS-PAGE and similar isoelectric point. Rat C8 differed from human C8 in the molecular weight of the gamma chain (23,000 and 21,000 kD respectively), and on isoelectric focusing (pI rat C8: 6.5-6.9; pI human C8: 7.4-7.9).

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Year:  1990        PMID： 2323185     DOI： 10.1159/000463125

Source DB:  PubMed          Journal:  Complement Inflamm        ISSN： 1012-8204


  7 in total

1.  Production and functional analysis of rat CD59 and chimeric CD59-Crry as active soluble proteins in Pichia pastoris.

Authors:  R J Quigg; C He; B K Hack; J J Alexander; B P Morgan
Journal:  Immunology       Date:  2000-01       Impact factor: 7.397

2.  Comparison of the suppressive effects of soluble CR1 and C5a receptor antagonist in acute arthritis induced in rats by blocking of CD59.

Authors:  M Mizuno; K Nishikawa; B P Morgan; S Matsuo
Journal:  Clin Exp Immunol       Date:  2000-02       Impact factor: 4.330

3.  Antibody-mediated demyelination in experimental allergic encephalomyelitis is independent of complement membrane attack complex formation.

Authors:  S Piddlesden; H Lassmann; I Laffafian; B P Morgan; C Linington
Journal:  Clin Exp Immunol       Date:  1991-02       Impact factor: 4.330

4.  Binding of human and rat CD59 to the terminal complement complexes.

Authors:  T Lehto; B P Morgan; S Meri
Journal:  Immunology       Date:  1997-01       Impact factor: 7.397

5.  Thyroid follicular cell function after non-lethal complement membrane attack.

Authors:  A P Weetman; M Freeman; B P Morgan
Journal:  Clin Exp Immunol       Date:  1990-10       Impact factor: 4.330

6.  CD59 or C3 are not requred for angiotensin II-dependent hypertension or hypertrophy in mice.

Authors:  Barbara Coles; Ruth Lewis; Peter B Anning; Jonathan Morton; Sivasankar Baalasubramanian; B Paul Morgan; Valerie B O'Donnell
Journal:  Immunology       Date:  2007-04-13       Impact factor: 7.397

7.  CD59a deficiency exacerbates influenza-induced lung inflammation through complement-dependent and -independent mechanisms.

Authors:  M Paula Longhi; Anwen Williams; Matthew Wise; B Paul Morgan; Awen Gallimore
Journal:  Eur J Immunol       Date:  2007-05       Impact factor: 5.532
  7 in total

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