PURPOSE: Small nucleolar RNAs (snoRNAs) direct sequence-specific modifications to ribosomal RNA. We hypothesized that the expression of snoRNAs may be altered in leukemic cells. METHODS: The expression of snoRNAs was analyzed in various leukemic cell lines by massive parallel sequencing (SOLiD). Quantitative real-time PCR (RT-qPCR) was used to validate the expression profiles. RESULTS: Our results show characteristic differences in the expression patterns of snoRNAs between cell lines representing the main subgroups of leukemia, AML, pre-B-ALL and T-ALL, respectively. In RT-qPCR analyses, several snoRNAs were found to be differentially expressed in T-ALL as compared to pre-B-ALL cell lines. CONCLUSIONS: snoRNAs are differentially expressed in various leukemic cell lines and could, therefore, be potentially useful in the classification of leukemia subgroups.
PURPOSE: Small nucleolar RNAs (snoRNAs) direct sequence-specific modifications to ribosomal RNA. We hypothesized that the expression of snoRNAs may be altered in leukemic cells. METHODS: The expression of snoRNAs was analyzed in various leukemic cell lines by massive parallel sequencing (SOLiD). Quantitative real-time PCR (RT-qPCR) was used to validate the expression profiles. RESULTS: Our results show characteristic differences in the expression patterns of snoRNAs between cell lines representing the main subgroups of leukemia, AML, pre-B-ALL and T-ALL, respectively. In RT-qPCR analyses, several snoRNAs were found to be differentially expressed in T-ALL as compared to pre-B-ALL cell lines. CONCLUSIONS: snoRNAs are differentially expressed in various leukemic cell lines and could, therefore, be potentially useful in the classification of leukemia subgroups.
Authors: T Liuksiala; K J Teittinen; K Granberg; M Heinäniemi; M Annala; M Mäki; M Nykter; O Lohi Journal: Leukemia Date: 2013-08-27 Impact factor: 11.528
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Authors: Grigory A Stepanov; Julia A Filippova; Andrey B Komissarov; Elena V Kuligina; Vladimir A Richter; Dmitry V Semenov Journal: Biomed Res Int Date: 2015-04-28 Impact factor: 3.411
Authors: J A Filippova; G A Stepanov; D V Semenov; O A Koval; E V Kuligina; I V Rabinov; V A Richter Journal: Acta Naturae Date: 2015 Apr-Jun Impact factor: 1.845