Literature DB >> 23219982

In vivo detection and quantification of chemicals that enhance protein stability.

Tsinatkeab T Hailu1, Linda Foit, James C A Bardwell.   

Abstract

We have devised protein-folding sensors that link protein stability to TEM-1 β-lactamase activity. The addition of osmolytes and other compounds with chemical chaperone activity to the growth medium of bacteria containing these sensors increases β-lactamase activity up to 207-fold in a dose-dependent manner. This enables the rapid detection and sensitive quantification of compounds that enhance in vivo protein stability.
Copyright © 2012 Elsevier Inc. All rights reserved.

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Year:  2012        PMID： 23219982      PMCID： PMC3670414          DOI： 10.1016/j.ab.2012.11.022

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  32 in total

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Authors:  Hiroshi Nikaido
Journal:  Microbiol Mol Biol Rev       Date:  2003-12       Impact factor: 11.056

2.  Chemical chaperones: mechanisms of action and potential use.

Authors:  E Papp; P Csermely
Journal:  Handb Exp Pharmacol       Date:  2006

3.  A molecular mechanism for osmolyte-induced protein stability.

Authors:  Timothy O Street; D Wayne Bolen; George D Rose
Journal:  Proc Natl Acad Sci U S A       Date:  2006-09-12       Impact factor: 11.205

Review 4.  Small molecule pharmacological chaperones: From thermodynamic stabilization to pharmaceutical drugs.

Authors:  Tsutomu Arakawa; Daisuke Ejima; Yoshiko Kita; Kouhei Tsumoto
Journal:  Biochim Biophys Acta       Date:  2006-09-01

5.  Three classes of glucocerebrosidase inhibitors identified by quantitative high-throughput screening are chaperone leads for Gaucher disease.

Authors:  Wei Zheng; Janak Padia; Daniel J Urban; Ajit Jadhav; Ozlem Goker-Alpan; Anton Simeonov; Ehud Goldin; Douglas Auld; Mary E LaMarca; James Inglese; Christopher P Austin; Ellen Sidransky
Journal:  Proc Natl Acad Sci U S A       Date:  2007-08-01       Impact factor: 11.205

6.  Glycerol increases the yield and activity of human phenylalanine hydroxylase mutant enzymes produced in a prokaryotic expression system.

Authors:  P Leandro; M C Lechner; I Tavares de Almeida; D Konecki
Journal:  Mol Genet Metab       Date:  2001-06       Impact factor: 4.797

7.  Efficient refolding of aggregation-prone citrate synthase by polyol osmolytes: how well are protein folding and stability aspects coupled?

Authors:  Rajesh Mishra; Robert Seckler; Rajiv Bhat
Journal:  J Biol Chem       Date:  2005-02-04       Impact factor: 5.157

8.  E. coli selection of human genes encoding secreted and membrane proteins based on cDNA fusions to a leaderless beta-lactamase reporter.

Authors:  Ruoying Tan; Xin Jiang; Alan Jackson; Pei Jin; Junming Yang; Ernestine Lee; Brendan Duggan; Laura L Stuve; Glenn K Fu
Journal:  Genome Res       Date:  2003-07-17       Impact factor: 9.043

9.  Chemical chaperone and inhibitor discovery: potential treatments for protein conformational diseases.

Authors:  Jian-Hua Zhao; Hsuan-Liang Liu; Hsin-Yi Lin; Chih-Hung Huang; Hsu-Wei Fang; Shiao-Shing Chen; Yih Ho; Wei-Bor Tsai; Wen-Yih Chen
Journal:  Perspect Medicin Chem       Date:  2007-12-11

10.  Optimizing protein stability in vivo.

Authors:  Linda Foit; Gareth J Morgan; Maximilian J Kern; Lenz R Steimer; Annekathrin A von Hacht; James Titchmarsh; Stuart L Warriner; Sheena E Radford; James C A Bardwell
Journal:  Mol Cell       Date:  2009-12-11       Impact factor: 17.970
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  9 in total

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Authors:  Zegbeh Z Kpadeh; Max Jameson-Lee; Anthony J Yeh; Olga Chertihin; Igor A Shumilin; Rafik Dey; Shandra R Day; Paul S Hoffman
Journal:  J Bacteriol       Date:  2013-02-22       Impact factor: 3.490

2.  Cytosolic selection systems to study protein stability.

Authors:  Ajamaluddin Malik; Antje Mueller-Schickert; James C A Bardwell
Journal:  J Bacteriol       Date:  2014-09-29       Impact factor: 3.490

3.  Probing the kinetic stabilities of Friedreich's ataxia clinical variants using a solid phase GroEL chaperonin capture platform.

Authors:  Ana R Correia; Subhashchandra Naik; Mark T Fisher; Cláudio M Gomes
Journal:  Biomolecules       Date:  2014-10-20

4.  Phytochemical analysis and anti-microbial activities of Artemisia spp. and rapid isolation methods of artemisinin.

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Journal:  AMB Express       Date:  2022-02-12       Impact factor: 3.298

5.  The effect of mutation on an aggregation-prone protein: An in vivo, in vitro, and in silico analysis.

Authors:  N Guthertz; R van der Kant; R M Martinez; Y Xu; C Trinh; B I Iorga; F Rousseau; J Schymkowitz; D J Brockwell; S E Radford
Journal:  Proc Natl Acad Sci U S A       Date:  2022-05-25       Impact factor: 12.779

6.  Super Spy variants implicate flexibility in chaperone action.

Authors:  Shu Quan; Lili Wang; Evgeniy V Petrotchenko; Karl At Makepeace; Scott Horowitz; Jianyi Yang; Yang Zhang; Christoph H Borchers; James Ca Bardwell
Journal:  Elife       Date:  2014-02-04       Impact factor: 8.140

7.  An in vivo platform for identifying inhibitors of protein aggregation.

Authors:  Janet C Saunders; Lydia M Young; Rachel A Mahood; Matthew P Jackson; Charlotte H Revill; Richard J Foster; D Alastair Smith; Alison E Ashcroft; David J Brockwell; Sheena E Radford
Journal:  Nat Chem Biol       Date:  2015-12-14       Impact factor: 15.040

Review 8.  Using protein engineering to understand and modulate aggregation.

Authors:  Jessica S Ebo; Nicolas Guthertz; Sheena E Radford; David J Brockwell
Journal:  Curr Opin Struct Biol       Date:  2020-02-19       Impact factor: 6.809

9.  An in vivo platform to select and evolve aggregation-resistant proteins.

Authors:  Jessica S Ebo; Janet C Saunders; Paul W A Devine; Alice M Gordon; Amy S Warwick; Bob Schiffrin; Stacey E Chin; Elizabeth England; James D Button; Christopher Lloyd; Nicholas J Bond; Alison E Ashcroft; Sheena E Radford; David C Lowe; David J Brockwell
Journal:  Nat Commun       Date:  2020-04-14       Impact factor: 14.919
  9 in total

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