Ke-Min Jin1, Min Lu, Fang-Fang Liu, Jin Gu, Xiao-Juan Du, Bao-Cai Xing. 1. Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Hepatic, Biliary & Pancreatic Surgery Unit I, Peking University Cancer Hospital & Institute, Beijing, China.
Abstract
BACKGROUND: Neural Wiskott-Aldrich syndrome protein (N-WASP) mediates migration and invasion in cancer cells, but its expression and clinicopathologic and prognostic importance in hepatocellular carcinoma (HCC) remain unknown. The present study was designed to address these issues. METHODS: N-WASP expression was first analyzed by Western blotting in 19 paired HCC and paratumoral liver (PTL) tissues. We further evaluated N-WASP expression immunohistochemically in samples from 119 patients with HCC. The clinicopathologic and prognostic importance of N-WASP expression were also investigated. RESULTS: Western blotting showed that N-WASP expression was up-regulated in 15 of 19 HCC tissues (79%), compared with PTL ones. The N-WASP-positive rate in immunohistochemical staining also was greater in HCC (63/119, 53%) than that in PTL tissues (8/119, 6%). The up-regulated N-WASP expression in HCC tissues was correlated with absence of capsule formation and predicted less overall and disease-free survival. Multivariate analysis demonstrated that N-WASP was an independent prognostic factor for overall survival and was marginally important for disease-free survival. CONCLUSION: These data establish that N-WASP is highly expressed in HCC and its strong prognostic importance. Therefore, the gene/protein might serve as a potential therapeutic target for HCC.
BACKGROUND:Neural Wiskott-Aldrich syndrome protein (N-WASP) mediates migration and invasion in cancer cells, but its expression and clinicopathologic and prognostic importance in hepatocellular carcinoma (HCC) remain unknown. The present study was designed to address these issues. METHODS:N-WASP expression was first analyzed by Western blotting in 19 paired HCC and paratumoral liver (PTL) tissues. We further evaluated N-WASP expression immunohistochemically in samples from 119 patients with HCC. The clinicopathologic and prognostic importance of N-WASP expression were also investigated. RESULTS: Western blotting showed that N-WASP expression was up-regulated in 15 of 19 HCC tissues (79%), compared with PTL ones. The N-WASP-positive rate in immunohistochemical staining also was greater in HCC (63/119, 53%) than that in PTL tissues (8/119, 6%). The up-regulated N-WASP expression in HCC tissues was correlated with absence of capsule formation and predicted less overall and disease-free survival. Multivariate analysis demonstrated that N-WASP was an independent prognostic factor for overall survival and was marginally important for disease-free survival. CONCLUSION: These data establish that N-WASP is highly expressed in HCC and its strong prognostic importance. Therefore, the gene/protein might serve as a potential therapeutic target for HCC.
Authors: Hayley T Morris; Loic Fort; Heather J Spence; Rachana Patel; David F Vincent; James H Park; Scott B Snapper; Francis A Carey; Owen J Sansom; Laura M Machesky Journal: J Pathol Date: 2018-05-28 Impact factor: 7.996
Authors: Esther García; Chiara Ragazzini; Xinzi Yu; Elena Cuesta-García; Jorge Bernardino de la Serna; Tobias Zech; David Sarrió; Laura M Machesky; Inés M Antón Journal: Sci Rep Date: 2016-03-24 Impact factor: 4.379
Authors: Amelie Juin; Heather J Spence; Kirsty J Martin; Ewan McGhee; Matthew Neilson; Marie F A Cutiongco; Nikolaj Gadegaard; Gillian Mackay; Loic Fort; Sergio Lilla; Gabriela Kalna; Peter Thomason; Yvette W H Koh; Jim C Norman; Robert H Insall; Laura M Machesky Journal: Dev Cell Date: 2019-10-24 Impact factor: 12.270