Sung-Whan Kim1, Hong-Zhe Zhang, Chae-Eun Kim, Jong-Min Kim, Moo Hyun Kim. 1. Regional Clinical Trial Center, Dong-A University Hospital, Busan, Korea; Department of Cardiology, College of Medicine, Dong-A University, Busan, Korea. Electronic address: sungwhk@yahoo.co.kr.
Abstract
BACKGROUND: We previously reported that amniotic mesenchymal stem cells (AMMs) possess high angio-vasulogenic properties. In this study, we investigated the chemotactic abilities of AMMs for improved cardiac function and regenerative angiogenesis. METHODS: The expressions of chemotactic and angiogenic genes were determined by qRT-PCR. Myocardial infarction (MI) was induced in NOD/SCID mice and cells were directly transplanted into the border regions of ischemic heart tissue. Immunohistochemical analysis was also conducted. RESULTS: AMMs significantly expressed the representative chemotactic factor GCP-2, NAP-2 as well as angiogenic factor Hif-1a. AMMs also highly expressed the chemokine receptors CCR2, CCR3 and CCR5. AMM transplantation improved left ventricular function, capillary density, angiogenic cytokine levels, angiopoetin (Ang)-1 and vascular endothelial growth factor (VEGF-A) levels in affected tissue. Immunohistochemical assaying also revealed increased engraftment and endothelial phenotypes. CONCLUSION: Our findings suggest that due to elevated survival and related chemotactic potential, AMMs are a promising stem cell source for the treatment of ischemic cardiovascular disease.
BACKGROUND: We previously reported that amniotic mesenchymal stem cells (AMMs) possess high angio-vasulogenic properties. In this study, we investigated the chemotactic abilities of AMMs for improved cardiac function and regenerative angiogenesis. METHODS: The expressions of chemotactic and angiogenic genes were determined by qRT-PCR. Myocardial infarction (MI) was induced in NOD/SCIDmice and cells were directly transplanted into the border regions of ischemic heart tissue. Immunohistochemical analysis was also conducted. RESULTS: AMMs significantly expressed the representative chemotactic factor GCP-2, NAP-2 as well as angiogenic factor Hif-1a. AMMs also highly expressed the chemokine receptors CCR2, CCR3 and CCR5. AMM transplantation improved left ventricular function, capillary density, angiogenic cytokine levels, angiopoetin (Ang)-1 and vascular endothelial growth factor (VEGF-A) levels in affected tissue. Immunohistochemical assaying also revealed increased engraftment and endothelial phenotypes. CONCLUSION: Our findings suggest that due to elevated survival and related chemotactic potential, AMMs are a promising stem cell source for the treatment of ischemic cardiovascular disease.
Authors: Annelies Bronckaers; Petra Hilkens; Yanick Fanton; Tom Struys; Pascal Gervois; Constantinus Politis; Wendy Martens; Ivo Lambrichts Journal: PLoS One Date: 2013-08-07 Impact factor: 3.240