| Literature DB >> 23218348 |
Tao Qin1, Jin Chen, Deyun Wang, Yuanliang Hu, Mi Wang, Jing Zhang, The Luong Nguyen, Cui Liu, Xu Liu.
Abstract
Chinese angelica polysaccharide (CAP) was extracted by water decoction and ethanol precipitation, purified through eliminating protein by Sevage method and column chromatography of Sephadex G-200, then selenizingly modified by nitric acid-sodium selenite method according to L(9)(3(4)) orthogonal design of three-factors, the usage amount of sodium selenite, reaction temperature and reaction time, at three level to obtain nine selenizing CAPs, sCAP(1)-sCAP(9). Their effects on chicken peripheral lymphocytes proliferation in vitro were compared by MTT assay taking the non-modified CAP as control. The results showed that selenylation modification could significantly enhance the immune-enhancing activity of CAP, sCAP(2) presented best effect and the optimal modification conditions were 200mg of sodium selenite for 500 mg of CAP, the reaction temperature of 70 °C and the reaction time of 6 h.Entities:
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Year: 2012 PMID： 23218348 DOI： 10.1016/j.carbpol.2012.08.097
Source DB: PubMed Journal: Carbohydr Polym ISSN： 0144-8617 Impact factor: 9.381