Literature DB >> 232177

Human cytomegalovirus-induced immediate early antigens: analysis in sodium dodecyl sulfate-polyacrylamide gel electrophoresis after immunoprecipitation.

S Michelson, F Horodniceanu, M Kress, M Tardy-Panit.   

Abstract

Immediate early antigen (IEA) induced in human lung fibroblasts by human cytomegalovirus was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis after immunoprecipitation with IEA-positive human sera. Two polypeptides of 76,000 daltons (76K) and 82K were detectable within 90 min after infection. Polypeptides of similar molecular weight were also found in immunoprecipitates of human cytomegalovirus-infected cells nonpermissive for virus replication. IEA is located within the nucleus, although some of the 76K material appears to be located on the outer nuclear membrane. Raising salt concentrations in the extraction buffer increased antigen extraction. The contribution of these IEA polypeptides to IEA nuclear fluorescent staining is discussed.

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Year:  1979        PMID： 232177      PMCID： PMC353550     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  19 in total

1.  Development of early nuclear antigen in cytomegalovirus infected cells in the presence of RNA and protein synthesis inhibitors.

Authors:  D W Reynolds
Journal:  J Gen Virol       Date:  1978-08       Impact factor: 3.891

2.  Chromatin pattern consisting of repeating bipartite structures in WI-38 cells infected with human cytomegalovirus.

Authors:  A L Kierszenbaum; E S Huang
Journal:  J Virol       Date:  1978-11       Impact factor: 5.103

3.  Human cytomegalovirus stimulates host cell RNA synthesis.

Authors:  S Tanaka; T Furukawa; S A Plotkin
Journal:  J Virol       Date:  1975-02       Impact factor: 5.103

4.  Synthesis of proteins and glycoproteins in cells infected with human cytomegalovirus.

Authors:  M F Stinski
Journal:  J Virol       Date:  1977-09       Impact factor: 5.103

5.  Growth of cytomegalovirus at supra-optimal temperatures.

Authors:  S Michelson-Fiske; J Arnoult; H Febvre
Journal:  J Gen Virol       Date:  1977-09       Impact factor: 3.891

6.  Cytomegalovirus-induced membrane antigens in productively infected cells.

Authors:  I Boldogh; E Gönczöl; L Váczi
Journal:  Acta Microbiol Acad Sci Hung       Date:  1977

7.  Modification of simian virus 40 protein A.

Authors:  P Tegtmeyer; K Rundell; J K Collins
Journal:  J Virol       Date:  1977-02       Impact factor: 5.103

8.  Immediate early antigens in human cytomegalovirus infected cells.

Authors:  S Michelson-Fiske; F Horodniceanu; J C Guillon
Journal:  Nature       Date:  1977-12-15       Impact factor: 49.962

9.  Sequence of protein synthesis in cells infected by human cytomegalovirus: early and late virus-induced polypeptides.

Authors:  M F Stinski
Journal:  J Virol       Date:  1978-06       Impact factor: 5.103

10.  Evidence for early nuclear antigens in cytomegalovirus-infected cells.

Authors:  L Geder
Journal:  J Gen Virol       Date:  1976-08       Impact factor: 3.891
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  13 in total

1.  Transactivation of a human cytomegalovirus early promoter by gene products from the immediate-early gene IE2 and augmentation by IE1: mutational analysis of the viral proteins.

Authors:  C L Malone; D H Vesole; M F Stinski
Journal:  J Virol       Date:  1990-04       Impact factor: 5.103

2.  Characterization of the major immediate-early polypeptides encoded by murine cytomegalovirus.

Authors:  G M Keil; M R Fibi; U H Koszinowski
Journal:  J Virol       Date:  1985-05       Impact factor: 5.103

3.  The human cytomegalovirus 86-kilodalton immediate-early 2 protein: synthesis as a precursor polypeptide and interaction with a 75-kilodalton protein of probable viral origin.

Authors:  L A Samaniego; M J Tevethia; D J Spector
Journal:  J Virol       Date:  1994-02       Impact factor: 5.103

4.  Expression of herpes simplex virus type I thymidine kinase gene in Escherichia coli.

Authors:  A C Garapin; F Colbère-Garapin; M Cohen-Solal; F Horodniceanu; P Kourilsky
Journal:  Proc Natl Acad Sci U S A       Date:  1981-02       Impact factor: 11.205

5.  Predominant immediate-early transcripts of human cytomegalovirus AD 169.

Authors:  G Jahn; E Knust; H Schmolla; T Sarre; J A Nelson; J K McDougall; B Fleckenstein
Journal:  J Virol       Date:  1984-02       Impact factor: 5.103

6.  Identification of polypeptide components of the Epstein-Barr virus early antigen complex with monoclonal antibodies.

Authors:  G R Pearson; B Vroman; B Chase; T Sculley; M Hummel; E Kieff
Journal:  J Virol       Date:  1983-07       Impact factor: 5.103

Review 7.  Assessment of human cytomegalovirus antibody detection techniques.

Authors:  F Horodniceanu; S Michelson
Journal:  Arch Virol       Date:  1980       Impact factor: 2.574

8.  Establishment of a rat cell line inducible for the expression of human cytomegalovirus immediate-early gene products by protein synthesis inhibition.

Authors:  R Boom; J L Geelen; C J Sol; A K Raap; R P Minnaar; B P Klaver; J van der Noordaa
Journal:  J Virol       Date:  1986-06       Impact factor: 5.103

9.  Studies on rat cytomegalovirus induced structural and non-structural proteins present at (immediate-)early and late times of infection.

Authors:  H Meijer; P H Dormans; C P van Boven
Journal:  Arch Virol       Date:  1986       Impact factor: 2.574

10.  Selective interference with class I major histocompatibility complex presentation of the major immediate-early protein following infection with human cytomegalovirus.

Authors:  M J Gilbert; S R Riddell; C R Li; P D Greenberg
Journal:  J Virol       Date:  1993-06       Impact factor: 5.103
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