Tong-ming Li1, Min-zhen Deng. 1. Guangzhou University of Chinese Medicine, School of Chinese Materia Medica, Guangzhou 510006, China. ltmwgx@yahoo.com.cn
Abstract
OBJECTIVE: To study the effect of Dendrobium mixture on hypoglycemic and the apoptosis of islet in rats with type 2 diabetic mellitus. METHODS: Type 2 diabetes mellitus models were induced by high sugar and fat diet and low dose intraperitoneal injection of streptozotocin (STZ) in rats, and treated with Dendrobium mixture (5, 10, 20 g/kg) by intragastric administration. Observed islet cell morphology with histopathological techniques and tested the apoptosis of islet cells by MTT and Annexin V/PI method. CONCLUSION: Dendrobium mixture could reduce the levels of blood glucose, triglyceride and glucosylated serum protein effectively and significantly improve the modeling structure and function of rat pancreatic tissue. The apoptotic islet cells was significantly reduced (P < 0.01) in treatment group compared with the model group. RESULTS: Dendrobium mixture have a hypoglycemic effect on rat models of type 2 diabetes. It can protect and restore the structure and function of pancreatic tissue.
OBJECTIVE: To study the effect of Dendrobium mixture on hypoglycemic and the apoptosis of islet in rats with type 2 diabetic mellitus. METHODS: Type 2 diabetes mellitus models were induced by high sugar and fat diet and low dose intraperitoneal injection of streptozotocin (STZ) in rats, and treated with Dendrobium mixture (5, 10, 20 g/kg) by intragastric administration. Observed islet cell morphology with histopathological techniques and tested the apoptosis of islet cells by MTT and Annexin V/PI method. CONCLUSION:Dendrobium mixture could reduce the levels of blood glucose, triglyceride and glucosylated serum protein effectively and significantly improve the modeling structure and function of rat pancreatic tissue. The apoptotic islet cells was significantly reduced (P < 0.01) in treatment group compared with the model group. RESULTS:Dendrobium mixture have a hypoglycemic effect on rat models of type 2 diabetes. It can protect and restore the structure and function of pancreatic tissue.