AIM: To investigate the role of delta-like ligand 4 (DLL4) in the angiogenesis of high-grade malignant glioma. MATERIALS AND METHODS: DLL4 expression and microvessel density (MVD) were detected by immunohistochemistry in 51 human high-grade malignant glioma tissue samples. The vessel maturation index (VMI) was calculated as the percentage of a-smooth muscle actin (a-SMA)-positive vessels in relation to the amount of CD31-positive vessels. Double fluorescent immunostaining for CD31 and EphrinB2 or EphB4 was performed to identify the arterial (EphrinB2) or venous (EphB4) origins of glioma microvessels. RESULTS: Strong immunostaining of DLL4 and a positive correlation of DLL4 with the MVD were observed in high-grade malignant gliomas. The VMI of the DLL4-positive group was significantly higher than that of the DLL4-negative group. However, no significant association was found between DLL4 and EphrinB2 or EphB4 in high-grade gliomas. CONCLUSION: DLL4 may be an important regulator for vessel proliferation and maturation in human high-grade malignant gliomas.
AIM: To investigate the role of delta-like ligand 4 (DLL4) in the angiogenesis of high-grade malignant glioma. MATERIALS AND METHODS:DLL4 expression and microvessel density (MVD) were detected by immunohistochemistry in 51 human high-grade malignant glioma tissue samples. The vessel maturation index (VMI) was calculated as the percentage of a-smooth muscle actin (a-SMA)-positive vessels in relation to the amount of CD31-positive vessels. Double fluorescent immunostaining for CD31 and EphrinB2 or EphB4 was performed to identify the arterial (EphrinB2) or venous (EphB4) origins of glioma microvessels. RESULTS: Strong immunostaining of DLL4 and a positive correlation of DLL4 with the MVD were observed in high-grade malignant gliomas. The VMI of the DLL4-positive group was significantly higher than that of the DLL4-negative group. However, no significant association was found between DLL4 and EphrinB2 or EphB4 in high-grade gliomas. CONCLUSION:DLL4 may be an important regulator for vessel proliferation and maturation in human high-grade malignant gliomas.
Authors: Eggehard Holler; Tao Sun; Rameshwar Patil; Anna Galstyan; Dmytro Klymyshyn; Hui Ding; Alexandra Chesnokova; Webster K Cavenee; Frank B Furnari; Vladimir A Ljubimov; Ekaterina S Shatalova; Shawn Wagner; Debiao Li; Adam N Mamelak; Serguei I Bannykh; Chirag G Patil; Jeremy D Rudnick; Jethro Hu; Zachary B Grodzinski; Arthur Rekechenetskiy; Vida Falahatian; Alexander V Lyubimov; Yongmei L Chen; Lai S Leoh; Tracy R Daniels-Wells; Manuel L Penichet; Alexander V Ljubimov; Keith L Black; Julia Y Ljubimova Journal: Cancer Res Date: 2019-01-18 Impact factor: 12.701
Authors: Bin Zhou; Hui Wang; Ren Liu; Mengzhe Wang; Huaifu Deng; Benjamin C Giglio; Parkash S Gill; Hong Shan; Zibo Li Journal: Mol Pharm Date: 2015-08-28 Impact factor: 4.939