BACKGROUND: Affinity and clonality of allergen-specific IgE antibodies are important determinants for the magnitude of IgE-mediated allergic inflammation. OBJECTIVE: We sought to analyze the contribution of heavy and light chains of human allergen-specific IgE antibodies for allergen specificity and to test whether promiscuous pairing of heavy and light chains with different allergen specificity allows binding and might affect affinity. METHODS: Ten IgE Fabs specific for 3 non-cross-reactive major timothy grass pollen allergens (Phl p 1, Phl p 2, and Phl p 5) obtained by means of combinatorial cloning from patients with grass pollen allergy were used to construct stable recombinant single chain variable fragments (ScFvs) representing the original Fabs and shuffled ScFvs in which heavy chains were recombined with light chains from IgE Fabs with specificity for other allergens by using the pCANTAB 5 E expression system. Possible ancestor genes for the heavy chain and light chain variable region-encoding genes were determined by using sequence comparison with the ImMunoGeneTics database, and their chromosomal locations were determined. Recombinant ScFvs were tested for allergen specificity and epitope recognition by means of direct and sandwich ELISA, and affinity by using surface plasmon resonance experiments. RESULTS: The shuffling experiments demonstrate that promiscuous pairing of heavy and light chains is possible and maintains allergen specificity, which is mainly determined by the heavy chains. ScFvs consisting of different heavy and light chains exhibited different affinities and even epitope specificity for the corresponding allergen. CONCLUSION: Our results indicate that allergen specificity of allergen-specific IgE is mainly determined by the heavy chains. Different heavy and light chain pairings in allergen-specific IgE antibodies affect affinity and epitope specificity and thus might influence clinical reactivity to allergens.
BACKGROUND: Affinity and clonality of allergen-specific IgE antibodies are important determinants for the magnitude of IgE-mediated allergic inflammation. OBJECTIVE: We sought to analyze the contribution of heavy and light chains of human allergen-specific IgE antibodies for allergen specificity and to test whether promiscuous pairing of heavy and light chains with different allergen specificity allows binding and might affect affinity. METHODS: Ten IgE Fabs specific for 3 non-cross-reactive major timothy grass pollen allergens (Phl p 1, Phl p 2, and Phl p 5) obtained by means of combinatorial cloning from patients with grass pollen allergy were used to construct stable recombinant single chain variable fragments (ScFvs) representing the original Fabs and shuffled ScFvs in which heavy chains were recombined with light chains from IgE Fabs with specificity for other allergens by using the pCANTAB 5 E expression system. Possible ancestor genes for the heavy chain and light chain variable region-encoding genes were determined by using sequence comparison with the ImMunoGeneTics database, and their chromosomal locations were determined. Recombinant ScFvs were tested for allergen specificity and epitope recognition by means of direct and sandwich ELISA, and affinity by using surface plasmon resonance experiments. RESULTS: The shuffling experiments demonstrate that promiscuous pairing of heavy and light chains is possible and maintains allergen specificity, which is mainly determined by the heavy chains. ScFvs consisting of different heavy and light chains exhibited different affinities and even epitope specificity for the corresponding allergen. CONCLUSION: Our results indicate that allergen specificity of allergen-specific IgE is mainly determined by the heavy chains. Different heavy and light chain pairings in allergen-specific IgE antibodies affect affinity and epitope specificity and thus might influence clinical reactivity to allergens.
Authors: A Horst; N Hunzelmann; S Arce; M Herber; R A Manz; A Radbruch; R Nischt; J Schmitz; M Assenmacher Journal: Clin Exp Immunol Date: 2002-12 Impact factor: 4.330
Authors: Ulrika Andréasson; Sabine Flicker; Malin Lindstedt; Rudolf Valenta; Lennart Greiff; Magnus Korsgren; Carl A K Borrebaeck; Mats Ohlin Journal: J Mol Biol Date: 2006-08-14 Impact factor: 5.469
Authors: S Vrtala; W R Sperr; I Reimitzer; R van Ree; S Laffer; W D Müller; P Valent; K Lechner; H Rumpold; D Kraft Journal: J Immunol Date: 1993-11-01 Impact factor: 5.422
Authors: J Eckl-Dorna; I Pree; J Reisinger; K Marth; K-W Chen; S Vrtala; S Spitzauer; R Valenta; V Niederberger Journal: Clin Exp Allergy Date: 2012-09 Impact factor: 5.018
Authors: M Levin; S Rotthus; S Wendel; N Najafi; E Källström; M Focke-Tejkl; R Valenta; S Flicker; M Ohlin Journal: Clin Exp Allergy Date: 2014-11 Impact factor: 5.018
Authors: Christoph Madritsch; Elisabeth Gadermaier; Uwe W Roder; Christian Lupinek; Rudolf Valenta; Sabine Flicker Journal: J Immunol Date: 2015-01-30 Impact factor: 5.422