AIMS: The lack of understanding of the biology of bone cancer metastasis has limited the development of effective treatment strategies. The aim of this study was to characterize tumor cell adhesion molecules and determine active tumor cell interactions with human bone marrow endothelial (BME) cells using atomic force microscopy. MATERIALS & METHODS: A single prostate (PC3) cancer cell was coupled (concanavalin A) to the atomic force microscopy cantilever then placed in contact with BME cells for cell force spectroscopy measurements. RESULTS & DISCUSSION: Strong adhesive interactions between PC3 and BME cells were significantly (p < 0.05) reduced by anti-ICAM-1, anti-β1 and anti-P-selectin, but not anti-VCAM-1. The combined blocking antibodies or the therapeutic agent zoledronic acid significantly (p < 0.005) reduced the adhesive interactions by 65 and 63%, respectively, which was confirmed using a functional in vitro assay. CONCLUSION: Atomic force microscopy provides a highly sensitive screening assay to determine and quantify nanoscale adhesion events between different cell types important in the metastatic cascade.
AIMS: The lack of understanding of the biology of bone cancer metastasis has limited the development of effective treatment strategies. The aim of this study was to characterize tumor cell adhesion molecules and determine active tumor cell interactions with human bone marrow endothelial (BME) cells using atomic force microscopy. MATERIALS & METHODS: A single prostate (PC3) cancer cell was coupled (concanavalin A) to the atomic force microscopy cantilever then placed in contact with BME cells for cell force spectroscopy measurements. RESULTS & DISCUSSION: Strong adhesive interactions between PC3 and BME cells were significantly (p < 0.05) reduced by anti-ICAM-1, anti-β1 and anti-P-selectin, but not anti-VCAM-1. The combined blocking antibodies or the therapeutic agent zoledronic acid significantly (p < 0.005) reduced the adhesive interactions by 65 and 63%, respectively, which was confirmed using a functional in vitro assay. CONCLUSION: Atomic force microscopy provides a highly sensitive screening assay to determine and quantify nanoscale adhesion events between different cell types important in the metastatic cascade.
Authors: Kamila Duś-Szachniewicz; Sławomir Drobczyński; Marta Woźniak; Krzysztof Zduniak; Katarzyna Ostasiewicz; Piotr Ziółkowski; Aleksandra K Korzeniewska; Anil K Agrawal; Paweł Kołodziej; Kinga Walaszek; Zbigniew Bystydzieński; Grzegorz Rymkiewicz Journal: Sci Rep Date: 2019-07-08 Impact factor: 4.379
Authors: Leike Xie; Zhe Sun; Nicola J Brown; Olga V Glinskii; Gerald A Meininger; Vladislav V Glinsky Journal: PLoS One Date: 2022-06-06 Impact factor: 3.752
Authors: Leike Xie; Zhe Sun; Zhongkui Hong; Nicola J Brown; Olga V Glinskii; Kate Rittenhouse-Olson; Gerald A Meininger; Vladislav V Glinsky Journal: PLoS One Date: 2018-09-20 Impact factor: 3.240