Ghada M Suddek1, Nora A Ashry, Nariman M Gameil. 1. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt, ghmsuddek@yahoo.com.
Abstract
OBJECTIVE: Antioxidant therapy may be useful in diseases with impaired oxidant-antioxidant balance. This study was designed to examine the effects of thymoquinone (TQ), an anti-inflammatory, antioxidant agent against cyclophosphamide (CP)-induced pulmonary oxidative damage. MATERIALS AND METHODS: Male Sprague-Dawley rats were categorized into four groups. Group I was control. Group II received TQ (100 mg/kg/day, p.o.) for 14 consecutive days. Group III was injected once with CP (150 mg/kg, i.p.). Group IV received TQ for 7 consecutive days, before and after CP injection. The parameters of study were tissue oxidant/antioxidant biomarkers and histological changes in rat lungs. RESULTS: A single intraperitoneal injection of CP markedly altered the levels of several biomarkers in lung homogenates. Significant increases in the content of lipid peroxides in lung were seen that paralleled the decreased levels of reduced glutathione. Cyclophosphamide increased the level of serum biomarkers: total protein, lactate dehydrogenase, and tumor necrosis factor-alpha (TNF-α). Treatment of rats with TQ 7 days before and after cyclophosphamide injection significantly attenuated the alterations in lung and serum biomarkers associated with inflammatory reactions, with less lipid peroxidation and restoration of antioxidants. Moreover, TQ attenuated the secretion of pro-inflammatory cytokine, TNF-α in rat serum. In addition, TQ effectively alleviated CP-induced histopathological changes in lung tissue. DISCUSSION AND CONCLUSION: Our results suggest that TQ produces a protective mechanism against CP-induced pulmonary damage and suggest a role of oxidative stress and inflammation in the pathogenesis.
OBJECTIVE: Antioxidant therapy may be useful in diseases with impaired oxidant-antioxidant balance. This study was designed to examine the effects of thymoquinone (TQ), an anti-inflammatory, antioxidant agent against cyclophosphamide (CP)-induced pulmonary oxidative damage. MATERIALS AND METHODS: Male Sprague-Dawley rats were categorized into four groups. Group I was control. Group II received TQ (100 mg/kg/day, p.o.) for 14 consecutive days. Group III was injected once with CP (150 mg/kg, i.p.). Group IV received TQ for 7 consecutive days, before and after CP injection. The parameters of study were tissue oxidant/antioxidant biomarkers and histological changes in rat lungs. RESULTS: A single intraperitoneal injection of CP markedly altered the levels of several biomarkers in lung homogenates. Significant increases in the content of lipid peroxides in lung were seen that paralleled the decreased levels of reduced glutathione. Cyclophosphamide increased the level of serum biomarkers: total protein, lactate dehydrogenase, and tumor necrosis factor-alpha (TNF-α). Treatment of rats with TQ 7 days before and after cyclophosphamide injection significantly attenuated the alterations in lung and serum biomarkers associated with inflammatory reactions, with less lipid peroxidation and restoration of antioxidants. Moreover, TQ attenuated the secretion of pro-inflammatory cytokine, TNF-α in rat serum. In addition, TQ effectively alleviated CP-induced histopathological changes in lung tissue. DISCUSSION AND CONCLUSION: Our results suggest that TQ produces a protective mechanism against CP-induced pulmonary damage and suggest a role of oxidative stress and inflammation in the pathogenesis.
Authors: Mohamed H Daba; Abdel-Aziz H Abdel-Aziz; Adel M Moustafa; Abdulhakeem A Al-Majed; Othman A Al-Shabanah; Hassan A El-Kashef Journal: Pharmacol Res Date: 2002-06 Impact factor: 7.658
Authors: Prashant R Gore; Chaitali P Prajapati; Umesh B Mahajan; Sameer N Goyal; Sateesh Belemkar; Shreesh Ojha; Chandragouda R Patil Journal: Int J Biol Sci Date: 2016-06-07 Impact factor: 6.580
Authors: Mohammad A Alzohairy; Amjad Ali Khan; Mohammed A Alsahli; Saleh A Almatroodi; Arshad Husain Rahmani Journal: Molecules Date: 2021-05-27 Impact factor: 4.411
Authors: Sameer N Goyal; Chaitali P Prajapati; Prashant R Gore; Chandragouda R Patil; Umesh B Mahajan; Charu Sharma; Sandhya P Talla; Shreesh K Ojha Journal: Front Pharmacol Date: 2017-09-21 Impact factor: 5.810
Authors: Naif A Al-Gabri; Sultan A M Saghir; Sallah A Al-Hashedi; Ali H El-Far; Asmaa F Khafaga; Ayman A Swelum; Abdullah S Al-Wajeeh; Shaker A Mousa; Mohamed E Abd El-Hack; Mohammed A E Naiel; Khaled A El-Tarabily Journal: Int J Nanomedicine Date: 2021-07-27