Zun-Zheng Wei1, Lai-Bo Luo, Hua-Li Zhang, Min Xiong, Xian Wang, Di Zhou. 1. Beijing Vegetable Research Center, National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, People's Republic of China.
Abstract
PREMISE OF THE STUDY: A new set of microsatellite or simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) was developed for arum lily (Zantedeschia aethiopica), which is one of the most iconic and widely recognized ornamental plants in the world. • METHODS AND RESULTS: Using 2175 unigenes derived from 4283 random ESTs in arum lily, 166 primer pairs were designed and tested for amplification in 24 accessions from Asia, Europe, and Africa. A total of 43 loci were polymorphic, with the number of alleles per locus ranging from two to 10. The observed heterozygosity, expected heterozygosity, and polymorphism information content ranged from 0.2313 to 0.8480, 0.3034 to 0.8648, and 0.1015 to 0.7364, respectively. • CONCLUSIONS: These novel polymorphic EST-SSR markers will facilitate future studies of genetic variation and molecular-assisted breeding systems in arum lily.
PREMISE OF THE STUDY: A new set of microsatellite or simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) was developed for arum lily (Zantedeschia aethiopica), which is one of the most iconic and widely recognized ornamental plants in the world. • METHODS AND RESULTS: Using 2175 unigenes derived from 4283 random ESTs in arum lily, 166 primer pairs were designed and tested for amplification in 24 accessions from Asia, Europe, and Africa. A total of 43 loci were polymorphic, with the number of alleles per locus ranging from two to 10. The observed heterozygosity, expected heterozygosity, and polymorphism information content ranged from 0.2313 to 0.8480, 0.3034 to 0.8648, and 0.1015 to 0.7364, respectively. • CONCLUSIONS: These novel polymorphic EST-SSR markers will facilitate future studies of genetic variation and molecular-assisted breeding systems in arum lily.
Authors: Elizabete de Souza Cândido; Gabriel da Rocha Fernandes; Sérgio Amorim de Alencar; Marlon Henrique e Silva Cardoso; Stella Maris de Freitas Lima; Vívian de Jesus Miranda; William Farias Porto; Diego Oliveira Nolasco; Nelson Gomes de Oliveira-Júnior; Aulus Estevão Anjos de Deus Barbosa; Robert Edward Pogue; Taia Maria Berto Rezende; Simoni Campos Dias; Octávio Luiz Franco Journal: PLoS One Date: 2014-03-10 Impact factor: 3.240