Plant Regeneration from Cultured Disc Florets of Tagetes erecta L.

The immature unpollinated disc florets showed differentiation of a large number of shoot primordia on MS (Murashige and Skoog, 1962) basal medium supplemented with BAP (5 mg · l(-1)) + IAA (3 mg · l(-1)). These shoot buds were further grown on a medium with BAP (3 mg · l(-1)) + IAA (5 mg · l(-1)) + GA(3) (0.5 mg · l(-1)) where they grew to a well developed shoot system. Complete plantlets were obtained by subculturing the shoots on a medium with IBA (0.5 mg · l(-1)) + GA(3) (0.5 mg · l(-1)). Plant regeneration from callus tissue could be maintained for two years by subculturing the callus together with developing shoot buds.