Adventitious Bud Formation from Bulb-scale Explants of Lilium speciosum Thunb. in vitro Interacting effects of NAA, TIBA, wounding, and temperature.

The present study was performed to find out whether the effects of the factors NAA, TIBA, tissue-wounding, and temperature interact during the process of adventitious bud formation from bulb-scale explants of Lilium speciosum Thunb., cv. Rubrum nr. 10, in vitro. The results refer to the number of buds formed and the polarity of the regeneration sites. The effects of TIBA and wounding were similar and additive and depended on the presence of NAA in the nutrient medium. The influence of temperature depended on the presence of the auxin and on the degree of wounding of the scale tissue. The results are discussed with special attention to the low endogenous IAA level of bulbscale tissue and to a possible role of ethylene in the regeneration process.