Regeneration of Plants by Embryogenesis with Callus Cultures of Carum carvi L.

This report describes the regeneration of plants from callus cultures of caraway, Carum carvi L. Callus of hypocotyl origin was maintained on medium containing Murashige and Skoog salts with Nitsch and Nitsch vitamins in the presence of 0.3 mg · l(-1) 2,4-D. Formation of somatic embryos was induced with suspension cultures once 2,4-D had been removed. Embryos developed into plantlets when subcultured on solid medium supplemented with 0.5 mg · l(-1) IBA and 10.0 mg · l(-1) adenine sulphate. Regenerated plantlets were transferred to soil.