BACKGROUND: As previously demonstrated, tumor associated macrophages (TAMs) infiltration is associated with some cancers invasion and metastasis. However, the role of TAMs in the gastric cancer remains unclear. METHODS: Three- dimensional dynamic migration imaging system and real time RT-PCR were used to quantitatively investigate the effect of macrophages on the cancer cell mobility and gene expression related to cancer invasion and metastasis, including ADAM8, ADAM9, MMP9, TIMP3, VEGF-A and IL8 genes, in AGS, HGC-27, Hs-746T and NCI-N87 gastric cancer cell lines under normal or hypoxic conditions. RESULTS: Under normal conditions, the cancer cell invasion rate was increased significantly and all six gene expressions were upregulated in all four cancer cell lines by macrophages. Under hypoxia the changes in the cancer cell invasion rate induced by macrophages was negatively correlated to the TIMP3 expression. In non- metastatic cell line AGS, the increase in migration rate induced by macrophages was further elevated under hypoxia with increased ADAM8 and ADAM9 expression and decreased MMP9 and TIMP3 expressions. Under hypoxia, the induction by macrophages for IL-8 expression was increased significantly in distant metastatic cell lines NCI-N87 and HS-746T, VEGF-A was increased in HGC-27 cell line. CONCLUSIONS: Both macrophages and hypoxia play an indispensable role in regulating the invasion of gastric cancer cells in vitro; ADAMs, MMP9 and TIMP3 might be involved in TAM induced invasive power of gastric cancer cells.
BACKGROUND: As previously demonstrated, tumor associated macrophages (TAMs) infiltration is associated with some cancers invasion and metastasis. However, the role of TAMs in the gastric cancer remains unclear. METHODS: Three- dimensional dynamic migration imaging system and real time RT-PCR were used to quantitatively investigate the effect of macrophages on the cancer cell mobility and gene expression related to cancer invasion and metastasis, including ADAM8, ADAM9, MMP9, TIMP3, VEGF-A and IL8 genes, in AGS, HGC-27, Hs-746T and NCI-N87 gastric cancer cell lines under normal or hypoxic conditions. RESULTS: Under normal conditions, the cancer cell invasion rate was increased significantly and all six gene expressions were upregulated in all four cancer cell lines by macrophages. Under hypoxia the changes in the cancer cell invasion rate induced by macrophages was negatively correlated to the TIMP3 expression. In non- metastatic cell line AGS, the increase in migration rate induced by macrophages was further elevated under hypoxia with increased ADAM8 and ADAM9 expression and decreased MMP9 and TIMP3 expressions. Under hypoxia, the induction by macrophages for IL-8 expression was increased significantly in distant metastatic cell lines NCI-N87 and HS-746T, VEGF-A was increased in HGC-27 cell line. CONCLUSIONS: Both macrophages and hypoxia play an indispensable role in regulating the invasion of gastric cancer cells in vitro; ADAMs, MMP9 and TIMP3 might be involved in TAM induced invasive power of gastric cancer cells.
Authors: Pauli Puolakkainen; Aino Koski; Sanna Vainionpää; Zhanlong Shen; Heikki Repo; Esko Kemppainen; Harri Mustonen; Hanna Seppänen Journal: Med Oncol Date: 2014-02-14 Impact factor: 3.064
Authors: Ana Rita Nobre; David Entenberg; Yarong Wang; John Condeelis; Julio A Aguirre-Ghiso Journal: Trends Cell Biol Date: 2018-07-23 Impact factor: 20.808
Authors: Ana P Cardoso; Marta L Pinto; Ana T Pinto; Marta T Pinto; Cátia Monteiro; Marta I Oliveira; Susana G Santos; João B Relvas; Raquel Seruca; Alberto Mantovani; Marc Mareel; Mário A Barbosa; Maria J Oliveira Journal: BMC Cancer Date: 2015-06-05 Impact factor: 4.430
Authors: Aino N E Salmiheimo; Harri K Mustonen; Sanna A A Vainionpää; Zhanlong Shen; Esko A J Kemppainen; Hanna E Seppänen; Pauli A Puolakkainen Journal: J Cancer Date: 2016-01-01 Impact factor: 4.207