Literature DB >> 23192043

Cloning, purification, crystallization and preliminary crystallographic study of calcium-binding protein 5 from Entamoeba histolytica.

Sanjeev Kumar1, Rana Zaidi, Samudrala Gourinath.   

Abstract

Entamoeba histolytica is the causative agent of human amoebiasis. Phagocytosis is the major route of food intake by this parasite and is responsible for its virulence. Calcium and calcium-binding proteins play major roles in its phagocytosis. Calcium-binding protein 5 from E. histolytica (EhCaBP5) is a cytoplasmic protein; its expression is very sensitive to serum starvation and it seems to be involved in binding to myosin I. In this study, EhCaBP5 was cloned, expressed in Escherichia coli and purified using affinity and size-exclusion chromatography. The purified protein crystallized in space group C222 and the crystals diffracted to 2 Å resolution. The Matthews coefficient indicated the presence of one molecule in the asymmetric unit, with a VM of 2.35 Å3 Da(-1) and a solvent content of 47.7%.

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Year:  2012        PMID： 23192043      PMCID： PMC3509984          DOI： 10.1107/S1744309112044612

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  13 in total

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Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2004-06-22

2.  Calcium binding protein 1 of the protozoan parasite Entamoeba histolytica interacts with actin and is involved in cytoskeleton dynamics.

Authors:  Nivedita Sahoo; Elisabeth Labruyère; Sudha Bhattacharya; P Sen; Nancy Guillén; Alok Bhattacharya
Journal:  J Cell Sci       Date:  2004-07-15       Impact factor: 5.285

3.  Crystal structure and trimer-monomer transition of N-terminal domain of EhCaBP1 from Entamoeba histolytica.

Authors:  Shivesh Kumar; Ejaz Ahmad; M Shahid Mansuri; Sanjeev Kumar; Ruchi Jain; Rizwan Hasan Khan; S Gourinath
Journal:  Biophys J       Date:  2010-06-16       Impact factor: 4.033

4.  The genome of the protist parasite Entamoeba histolytica.

Authors:  Brendan Loftus; Iain Anderson; Rob Davies; U Cecilia M Alsmark; John Samuelson; Paolo Amedeo; Paola Roncaglia; Matt Berriman; Robert P Hirt; Barbara J Mann; Tomo Nozaki; Bernard Suh; Mihai Pop; Michael Duchene; John Ackers; Egbert Tannich; Matthias Leippe; Margit Hofer; Iris Bruchhaus; Ute Willhoeft; Alok Bhattacharya; Tracey Chillingworth; Carol Churcher; Zahra Hance; Barbara Harris; David Harris; Kay Jagels; Sharon Moule; Karen Mungall; Doug Ormond; Rob Squares; Sally Whitehead; Michael A Quail; Ester Rabbinowitsch; Halina Norbertczak; Claire Price; Zheng Wang; Nancy Guillén; Carol Gilchrist; Suzanne E Stroup; Sudha Bhattacharya; Anuradha Lohia; Peter G Foster; Thomas Sicheritz-Ponten; Christian Weber; Upinder Singh; Chandrama Mukherjee; Najib M El-Sayed; William A Petri; C Graham Clark; T Martin Embley; Bart Barrell; Claire M Fraser; Neil Hall
Journal:  Nature       Date:  2005-02-24       Impact factor: 49.962

5.  Calmodulin-like protein from Entamoeba histolytica: solution structure and calcium-binding properties of a partially folded protein.

Authors:  Ashok K Rout; Narendra Padhan; R P Barnwal; A Bhattacharya; Kandala V R Chary
Journal:  Biochemistry       Date:  2010-12-21       Impact factor: 3.162

Review 6.  Calcium-binding proteins of Entamoeba histolytica.

Authors:  Alok Bhattacharya; Narendra Padhan; Ruchi Jain; Sudha Bhattacharya
Journal:  Arch Med Res       Date:  2006-02       Impact factor: 2.235

7.  Calcium-binding protein 1 of Entamoeba histolytica transiently associates with phagocytic cups in a calcium-independent manner.

Authors:  Ruchi Jain; Julien Santi-Rocca; Narendra Padhan; Sudha Bhattacharya; Nancy Guillen; Alok Bhattacharya
Journal:  Cell Microbiol       Date:  2008-03-11       Impact factor: 3.715

8.  Crystallization and preliminary crystallographic analysis of calcium-binding protein-2 from Entamoeba histolytica and its complexes with strontium and the IQ1 motif of myosin V.

Authors:  S Gourinath; Narendra Padhan; Neelima Alam; Alok Bhattacharya
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2005-04-01

9.  Crystal structure of calcium binding protein-1 from Entamoeba histolytica: a novel arrangement of EF hand motifs.

Authors:  Shivesh Kumar; Narendra Padhan; Neelima Alam; S Gourinath
Journal:  Proteins       Date:  2007-09-01

10.  N- and C-terminal domains of the calcium binding protein EhCaBP1 of the parasite Entamoeba histolytica display distinct functions.

Authors:  Ruchi Jain; Shivesh Kumar; Samudrala Gourinath; Sudha Bhattacharya; Alok Bhattacharya
Journal:  PLoS One       Date:  2009-04-22       Impact factor: 3.240
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  3 in total

Review 1.  Structural and functional diversity of Entamoeba histolytica calcium-binding proteins.

Authors:  Sanjeev Kumar; Shalini Mishra; S Gourinath
Journal:  Biophys Rev       Date:  2020-10-15

2.  Indirect Haemagglutination Test in Comparison with ELISA for Detection of Antibodies against Invasive Amoebiasis.

Authors:  Sankaramoorthy Dhanalakshmi; Chidambaram Meenachi; Subhash Chandra Parija
Journal:  J Clin Diagn Res       Date:  2016-08-01

3.  Crystal structure of calcium binding protein-5 from Entamoeba histolytica and its involvement in initiation of phagocytosis of human erythrocytes.

Authors:  Sanjeev Kumar; Saima Aslam; Mohit Mazumder; Pradeep Dahiya; Aruna Murmu; Babu A Manjasetty; Rana Zaidi; Alok Bhattacharya; S Gourinath
Journal:  PLoS Pathog       Date:  2014-12-11       Impact factor: 6.823
  3 in total

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