Literature DB >> 2318929

A new method for extracting DNA or RNA for polymerase chain reaction.

O Yamada1, T Matsumoto, M Nakashima, S Hagari, T Kamahora, H Ueyama, Y Kishi, H Uemura, T Kurimura.   

Abstract

The use of glass powder suspension for the extraction of RNA or DNA was studied to simplify the procedures of polymerase chain reaction (PCR). Using this procedure, proviral DNA of human T-lymphotropic virus type-1 (HTLV-1) in the blood of an asymptomatic virus carrier and viral RNA of human immunodeficiency virus (HIV) in the blood of an AIDS patient were easily detected by PCR employing glass powder. The use of glass powder is a simple and highly efficient procedure for the extraction of DNA or RNA, and can be applied for routine PCR.

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Year:  1990        PMID： 2318929     DOI： 10.1016/0166-0934(90)90136-4

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  7 in total

1.  Simultaneous detection of HBV and HCV by multiplex PCR normalization.

Authors:  Ning Wang; Xue-Qin Gao; Jin-Xiang Han
Journal:  World J Gastroenterol       Date:  2004-08-15       Impact factor: 5.742

2.  Development of a low-resource RNA extraction cassette based on surface tension valves.

Authors:  Hali Bordelon; Nicholas M Adams; Amy S Klemm; Patricia K Russ; John V Williams; H Keipp Talbot; David W Wright; Frederick R Haselton
Journal:  ACS Appl Mater Interfaces       Date:  2011-05-31       Impact factor: 9.229

3.  Detection of adenoviruses and enteroviruses in polluted waters by nested PCR amplification.

Authors:  M Puig; J Jofre; F Lucena; A Allard; G Wadell; R Girones
Journal:  Appl Environ Microbiol       Date:  1994-08       Impact factor: 4.792

4.  Identification of group A rotavirus gene 4 types by polymerase chain reaction.

Authors:  J R Gentsch; R I Glass; P Woods; V Gouvea; M Gorziglia; J Flores; B K Das; M K Bhan
Journal:  J Clin Microbiol       Date:  1992-06       Impact factor: 5.948

5.  Detection of mycoplasma contaminations by the polymerase chain reaction.

Authors:  M Wirth; E Berthold; M Grashoff; H Pfützner; U Schubert; H Hauser
Journal:  Cytotechnology       Date:  1994       Impact factor: 2.058

6.  Development of a method for detection of enteroviruses in shellfish by PCR with poliovirus as a model.

Authors:  D N Lees; K Henshilwood; W J Doré
Journal:  Appl Environ Microbiol       Date:  1994-08       Impact factor: 4.792

7.  Comparison of in-house SARS-CoV-2 genome extraction procedures. A need for COVID-19 pandemic.

Authors:  Gabriel Martín; Susana Rojo-Alba; Cristian Castelló-Abietar; Fátima Abreu-Salinas; Isabel Costales; Jose Antonio Boga; Santiago Melón; Marta Elena Álvarez-Argüelles
Journal:  J Virol Methods       Date:  2021-12-10       Impact factor: 2.014
  7 in total

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