Leila Beigom Hejazian1,2, Banafshe Esmaeilzade3, Fatima Moghanni Ghoroghi1, Fatemeh Moradi1, Marzieh Beigom Hejazian4, Anahita Aslani5, Mehrdad Bakhtiari1, Masoud Soleimani6, Maliheh Nobakht7. 1. Dept. of Histology and Neuroscience, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. 2. Dept. of Anatomy, School of Medicine, Babol University of Medical Science, Babol, Iran. 3. Dept. of Anatomy, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran. 4. Physical Education and Sports Sciences, Dept. of Physical Education, Payame Noor University of Tehran, Tehran, Iran. 5. Microscopic Surgen, Minimally Invasive Surgery Research Center, Rassoul Akram Hospital, Tehran University of Medical Science, Tehran, Iran. 6. 6Dept. of Hematology, School of Medicine, Tarbiat Modares University, Tehran, Iran. 7. Anti-microbial Resistance Research Center, Tehran University of Medical Science, Tehran, Iran.
Abstract
BACKGROUND: The aim of this study was to fabricate the poly caprolactone (PCL) aligned nanofiber scaffold and to evaluate the survival, adhesion, proliferation, and differentiation of rat hair follicle stem cells (HFSC) in the graft material using electrospun PCL nanofiber scaffold for tissue engineering applications. METHODS: The bulge region of rat whisker was isolated and cultured in DMEM: nutrient mixture F-12 supplemented with epidermal growth factor. The morphological and biological features of cultured bulge cells were observed by light microscopy using immunocytochemistry methods. Electrospinning was used for production of PCL nanofiber scaffolds. Scanning electron microscopy (SEM), 3-(4, 5-di-methylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, and histology analysis were used to investigate the cell morphology, viability, attachment and infiltration of the HFSC on the PCL nanofiber scaffolds. RESULTS: The results of the MTT assay showed cell viability and cell proliferation of the HFSC on PCL nanofiber scaffolds. SEM microscopy images indicated that HFSC are attached, proliferated and spread on PCL nanofiber scaffolds. Also, immunocytochemical analysis showed cell infiltration and cell differentiation on the scaffolds. CONCLUSION: The results of this study reveal that PCL nanofiber scaffolds are suitable for cell culture, proliferation, differentiation and attachment. Furthermore, HFSC are attached and proliferated on PCL nanofiber scaffolds.
BACKGROUND: The aim of this study was to fabricate the poly caprolactone (PCL) aligned nanofiber scaffold and to evaluate the survival, adhesion, proliferation, and differentiation of rat hair follicle stem cells (HFSC) in the graft material using electrospun PCL nanofiber scaffold for tissue engineering applications. METHODS: The bulge region of rat whisker was isolated and cultured in DMEM: nutrient mixture F-12 supplemented with epidermal growth factor. The morphological and biological features of cultured bulge cells were observed by light microscopy using immunocytochemistry methods. Electrospinning was used for production of PCL nanofiber scaffolds. Scanning electron microscopy (SEM), 3-(4, 5-di-methylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, and histology analysis were used to investigate the cell morphology, viability, attachment and infiltration of the HFSC on the PCL nanofiber scaffolds. RESULTS: The results of the MTT assay showed cell viability and cell proliferation of the HFSC on PCL nanofiber scaffolds. SEM microscopy images indicated that HFSC are attached, proliferated and spread on PCL nanofiber scaffolds. Also, immunocytochemical analysis showed cell infiltration and cell differentiation on the scaffolds. CONCLUSION: The results of this study reveal that PCL nanofiber scaffolds are suitable for cell culture, proliferation, differentiation and attachment. Furthermore, HFSC are attached and proliferated on PCL nanofiber scaffolds.
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