Literature DB >> 23179827

The development of T lymphocytes in fetal thymus organ culture.

Takeshi Nitta1, Izumi Ohigashi, Yousuke Takahama.   

Abstract

Fetal thymus organ culture (FTOC) is a unique and powerful culture system that allows intrathymic T-lymphocyte development in vitro. T-cell development in FTOC well represents fetal thymocyte development in vivo. Here we describe the basic method for FTOC as well as several related techniques, including reconstitution of thymus lobes with T-lymphoid progenitor cells, high-oxygen submersion culture, reaggregation thymus organ culture, retrovirus-mediated gene transfer to developing thymocytes in FTOC, and coculture of progenitor cells with OP9-DL1 cells.

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Year:  2013        PMID： 23179827     DOI： 10.1007/978-1-62703-128-8_6

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  12 in total

1.  A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants.

Authors:  Raul Vizcardo; S M Rafiqul Islam; Takuya Maeda; Naritaka Tamaoki; Meghan L Good; Nicholas D Klemen; Marta Bosch-Marce; Li Jia; Mikhael J Kruhlak; Nicholas P Restifo
Journal:  J Vis Exp       Date:  2019-08-09       Impact factor: 1.355

2.  γδTCR recruits the Syk/PI3K axis to drive proinflammatory differentiation program.

Authors:  Ryunosuke Muro; Takeshi Nitta; Kenta Nakano; Tadashi Okamura; Hiroshi Takayanagi; Harumi Suzuki
Journal:  J Clin Invest       Date:  2017-12-04       Impact factor: 14.808

3.  Fibroblasts as a source of self-antigens for central immune tolerance.

Authors:  Takeshi Nitta; Masanori Tsutsumi; Sachiko Nitta; Ryunosuke Muro; Emma C Suzuki; Kenta Nakano; Yoshihiko Tomofuji; Shinichiro Sawa; Tadashi Okamura; Josef M Penninger; Hiroshi Takayanagi
Journal:  Nat Immunol       Date:  2020-08-24       Impact factor: 25.606

4.  Generation of Tumor Antigen-Specific iPSC-Derived Thymic Emigrants Using a 3D Thymic Culture System.

Authors:  Raul Vizcardo; Nicholas D Klemen; S M Rafiqul Islam; Devikala Gurusamy; Naritaka Tamaoki; Daisuke Yamada; Haruhiko Koseki; Benjamin L Kidder; Zhiya Yu; Li Jia; Amanda N Henning; Meghan L Good; Marta Bosch-Marce; Takuya Maeda; Chengyu Liu; Zied Abdullaev; Svetlana Pack; Douglas C Palmer; David F Stroncek; Fumito Ito; Francis A Flomerfelt; Michael J Kruhlak; Nicholas P Restifo
Journal:  Cell Rep       Date:  2018-03-20       Impact factor: 9.423

5.  The thymic cortical epithelium determines the TCR repertoire of IL-17-producing γδT cells.

Authors:  Takeshi Nitta; Ryunosuke Muro; Yukiko Shimizu; Sachiko Nitta; Hiroyo Oda; Yuki Ohte; Motohito Goto; Rieko Yanobu-Takanashi; Tomoya Narita; Hiroshi Takayanagi; Hisataka Yasuda; Tadashi Okamura; Shigeo Murata; Harumi Suzuki
Journal:  EMBO Rep       Date:  2015-03-13       Impact factor: 8.807

6.  Infection of adult thymus with murine retrovirus induces virus-specific central tolerance that prevents functional memory CD8+ T cell differentiation.

Authors:  Shiki Takamura; Eiji Kajiwara; Sachiyo Tsuji-Kawahara; Tomoko Masumoto; Makoto Fujisawa; Maiko Kato; Tomomi Chikaishi; Yuri Kawasaki; Saori Kinoshita; Manami Itoi; Nobuo Sakaguchi; Masaaki Miyazawa
Journal:  PLoS Pathog       Date:  2014-03-20       Impact factor: 6.823

7.  The Ras GTPase-activating protein Rasal3 supports survival of naive T cells.

Authors:  Ryunosuke Muro; Takeshi Nitta; Toshiyuki Okada; Hitoshi Ideta; Takeshi Tsubata; Harumi Suzuki
Journal:  PLoS One       Date:  2015-03-20       Impact factor: 3.240

8.  Soluble antigens from the neurotropic pathogen Angiostrongylus cantonensis directly induce thymus atrophy in a mouse model.

Authors:  Zhen Liu; Dong-Ming Su; Zi-Long Yu; Feng Wu; Rui-Feng Liu; Shi-Qi Luo; Zhi-Yue Lv; Xin Zeng; Xi Sun; Zhong-Dao Wu
Journal:  Oncotarget       Date:  2017-07-25

9.  Thymic homing of activated CD4+ T cells induces degeneration of the thymic epithelium through excessive RANK signaling.

Authors:  Chen Yin; Xiao-Yan Pei; Hui Shen; Ya-Nan Gao; Xiu-Yuan Sun; Wei Wang; Qing Ge; Yu Zhang
Journal:  Sci Rep       Date:  2017-05-25       Impact factor: 4.379

10.  Identification of a novel lymphoid population in the murine epidermis.

Authors:  Francisca F Almeida; Mari Tenno; Joanna Brzostek; Jackson LiangYao Li; Gabriele Allies; Guillaume Hoeffel; Peter See; Lai Guan Ng; Hans Jörg Fehling; Nicholas R J Gascoigne; Ichiro Taniuchi; Florent Ginhoux
Journal:  Sci Rep       Date:  2015-07-30       Impact factor: 4.379
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