AIM: The CD133 antigen has been identified as a putative stem cell marker in colorectal cancer tissues. The aim of this study was to investigate the cell cycle state of CD133(+) and CD133(-) cells, isolated from primary human colorectal tumors. MATERIALS AND METHODS: After mechanical and enzymatic dissociation of the tumor samples, CD133(+) and CD133(-) subsets were identified and separated by magnetic cell sorting. Flow cytometric analysis was performed to compare the cell cycle of both CD133(+) and CD133(-) cells isolated from primary and liver metastatic cancer cells. RESULTS: The results indicated that CD133(+) cells isolated from both primary and liver metastatic colorectal cancers were found in higher percentage in the G0/G1 phases. However, the CD133(-) cells isolated from primary colorectal cancers were predominantly found in the S and G2/M phases. Surprisingly, the CD133(-) cells isolated from liver metastatic colorectal cancers were mostly found in the G0/G1 phase. CONCLUSION: The present study provides evidence that CD133(+) cells are in a quiescent state in colorectal cancer, representing a mechanism that would at least partially explain chemotherapy resistance and tumor recurrence in post-therapy patients.
AIM: The CD133 antigen has been identified as a putative stem cell marker in colorectal cancer tissues. The aim of this study was to investigate the cell cycle state of CD133(+) and CD133(-) cells, isolated from primary humancolorectal tumors. MATERIALS AND METHODS: After mechanical and enzymatic dissociation of the tumor samples, CD133(+) and CD133(-) subsets were identified and separated by magnetic cell sorting. Flow cytometric analysis was performed to compare the cell cycle of both CD133(+) and CD133(-) cells isolated from primary and liver metastatic cancer cells. RESULTS: The results indicated that CD133(+) cells isolated from both primary and liver metastatic colorectal cancers were found in higher percentage in the G0/G1 phases. However, the CD133(-) cells isolated from primary colorectal cancers were predominantly found in the S and G2/M phases. Surprisingly, the CD133(-) cells isolated from liver metastatic colorectal cancers were mostly found in the G0/G1 phase. CONCLUSION: The present study provides evidence that CD133(+) cells are in a quiescent state in colorectal cancer, representing a mechanism that would at least partially explain chemotherapy resistance and tumor recurrence in post-therapy patients.
Authors: Naser Mobarra; Abbas Shafiee; Seyed Mohammad Ali Hosseini Rad; Nooshin Tasharrofi; Mina Soufi-Zomorod; Maryam Hafizi; Marjan Movahed; Fatemeh Kouhkan; Masoud Soleimani Journal: In Vitro Cell Dev Biol Anim Date: 2015-02-12 Impact factor: 2.416
Authors: Pavel Pitule; Miroslava Cedikova; Ondrej Daum; Jan Vojtisek; Ondrej Vycital; Petr Hosek; Vladislav Treska; Ondrej Hes; Milena Kralickova; Vaclav Liska Journal: Biomed Res Int Date: 2014-04-22 Impact factor: 3.411
Authors: Elizabeth Ann Roundhill; Mariona Chicon-Bosch; Lee Jeys; Michael Parry; Kenneth S Rankin; Alastair Droop; Susan Ann Burchill Journal: Cell Oncol (Dordr) Date: 2021-08-17 Impact factor: 6.730
Authors: Asmaa M Zahran; Amal Rayan; Hussein Fakhry; Alia M Attia; Ahmed M Ashmawy; Ahmed Soliman; Azza Elkady; Helal F Hetta Journal: Cancer Manag Res Date: 2019-02-07 Impact factor: 3.989