| Literature DB >> 2317380 |
K Yamagata1, K Goto, C H Kuo, H Kondo, N Miki.
Abstract
Visinin is a retinal cone cell-specific protein (molecular weight 24,000, pI 5.1). To investigate its function, visinin cDNA was isolated from a chick retinal lambda gt11 cDNA library, using anti-visinin serum. The beta-galactosidase-visinin fusion protein was used for purifying epitope-selected antibody. The purified visinin antibody reacted only with a 24 kd protein in retinal cone cells. Visinin mRNA was expressed only in the retinal photoreceptor layer. The nucleotide sequence of the cDNA revealed that visinin has three E-F hand structures and is a Ca2+ binding protein. Visinin protein expressed in E. coli exhibited Ca2+ binding activity. These results suggest that visinin is a photoreceptor-specific Ca2+ binding protein and may be involved in phototransduction in the cone cells.Entities:
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Year: 1990 PMID： 2317380 DOI： 10.1016/0896-6273(90)90059-o
Source DB: PubMed Journal: Neuron ISSN： 0896-6273 Impact factor: 17.173