Literature DB >> 2317213

Isolation and characterization of hyaluronidase from scorpion (Heterometrus fulvipes) venom.

M Ramanaiah1, P R Parthasarathy, B Venkaiah.   

Abstract

Hyaluronidase (Hyaluronate lyase, E.C. 3.2.1.35) has been isolated from Heterometrus fulvipes scorpion venom by a combination of gel filtration on Sephadex G-75 and ion exchange chromatography on DEAE-cellulose. The enzyme preparation showed a single band on polyacrylamide gel electrophoresis and a molecular weight of 82,000. The final preparation was purified 27-fold. The optimum pH for enzyme activity was 4.0. No loss of activity was observed up to 30 degrees C and showed a sharp decrease in activity at 50 degrees C. Heparin inhibited the enzyme activity.

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Year:  1990        PMID： 2317213

Source DB:  PubMed          Journal:  Biochem Int        ISSN： 0158-5231


  10 in total

Review 1.  Hyaluronidases--a group of neglected enzymes.

Authors:  G Kreil
Journal:  Protein Sci       Date:  1995-09       Impact factor: 6.725

2.  Identification of the reaction products of the purified hyaluronidase from stonefish (Synanceja horrida) venom.

Authors:  K Sugahara; S Yamada; M Sugiura; K Takeda; R Yuen; H E Khoo; C H Poh
Journal:  Biochem J       Date:  1992-04-01       Impact factor: 3.857

3.  Expression of hyaluronidase by tumor cells induces angiogenesis in vivo.

Authors:  D Liu; E Pearlman; E Diaconu; K Guo; H Mori; T Haqqi; S Markowitz; J Willson; M S Sy
Journal:  Proc Natl Acad Sci U S A       Date:  1996-07-23       Impact factor: 11.205

4.  Hyaluronidase significantly enhances the efficacy of regional vinblastine chemotherapy of malignant melanoma.

Authors:  T Spruss; G Bernhardt; H Schönenberger; W Schiess
Journal:  J Cancer Res Clin Oncol       Date:  1995       Impact factor: 4.553

5.  Inhibitory effects of flavonoids on several venom hyaluronidases.

Authors:  U R Kuppusamy; N P Das
Journal:  Experientia       Date:  1991-12-01

6.  The Cuban scorpion Rhopalurus junceus (Scorpiones, Buthidae): component variations in venom samples collected in different geographical areas.

Authors:  Rodolfo Rodríguez-Ravelo; Fredy I V Coronas; Fernando Z Zamudio; Lidia González-Morales; Georgina Espinosa López; Ariel Ruiz Urquiola; Lourival D Possani
Journal:  J Venom Anim Toxins Incl Trop Dis       Date:  2013-05-20

7.  Molecular, immunological, and biological characterization of Tityus serrulatus venom hyaluronidase: new insights into its role in envenomation.

Authors:  Carolina Campolina Rebello Horta; Bárbara de Freitas Magalhães; Bárbara Bruna Ribeiro Oliveira-Mendes; Anderson Oliveira do Carmo; Clara Guerra Duarte; Liza Figueiredo Felicori; Ricardo Andrez Machado-de-Ávila; Carlos Chávez-Olórtegui; Evanguedes Kalapothakis
Journal:  PLoS Negl Trop Dis       Date:  2014-02-13

8.  Arthropod venom Hyaluronidases: biochemical properties and potential applications in medicine and biotechnology.

Authors:  Karla C F Bordon; Gisele A Wiezel; Fernanda G Amorim; Eliane C Arantes
Journal:  J Venom Anim Toxins Incl Trop Dis       Date:  2015-10-22

Review 9.  Biotechnological Trends in Spider and Scorpion Antivenom Development.

Authors:  Andreas Hougaard Laustsen; Mireia Solà; Emma Christine Jappe; Saioa Oscoz; Line Præst Lauridsen; Mikael Engmark
Journal:  Toxins (Basel)       Date:  2016-07-23       Impact factor: 4.546

10.  Pioneering Study on Rhopalurus crassicauda Scorpion Venom: Isolation and Characterization of the Major Toxin and Hyaluronidase.

Authors:  Caio B Abreu; Karla C F Bordon; Felipe A Cerni; Isadora S Oliveira; Carla Balenzuela; Gabriel M Alexandre-Silva; Karina F Zoccal; Mouzarllem B Reis; Gisele A Wiezel; Steve Peigneur; Ernesto L Pinheiro-Júnior; Jan Tytgat; Tiago M Cunha; Loic Quinton; Lúcia H Faccioli; Eliane C Arantes; Umberto Zottich; Manuela B Pucca
Journal:  Front Immunol       Date:  2020-08-20       Impact factor: 7.561
  10 in total

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