AIM: Current synthetic tubular conduits are inferior to nerve autograft for the repair of segmental peripheral nerve injuries. We examined motor outcomes with the use of longitudinally aligned poly (L-lactide-co-caprolactone) nanofiber conduits for repair of nerve gap injury in a rat model. METHODS: Ten-millimeter segments of sciatic nerve were resected in 44 Lewis rats. The gaps were either left unrepaired (n = 6), repaired with nerve autograft (n = 19), or repaired with conduit (n = 19). After 12 weeks, nerve conduction latency, compound muscle action potential amplitude, muscle force and muscle mass were measured. The numbers of axons and axon diameters both within the grafts and distally were determined. RESULTS: After 12 weeks, gastrocnemius isometric tetanic force and muscle mass for the conduit group reached 85 and 82% of autograft values, respectively. Nerve conduction and compound muscle action potential were not significantly different between these two groups, although the latter approached significance. There was no recovery in the unrepaired group. CONCLUSION: Muscle recovery for the animals treated with this aligned nanofiber conduit approached that of autograft, suggesting the importance of internal conduit structure for nerve repair.
AIM: Current synthetic tubular conduits are inferior to nerve autograft for the repair of segmental peripheral nerve injuries. We examined motor outcomes with the use of longitudinally aligned poly (L-lactide-co-caprolactone) nanofiber conduits for repair of nerve gap injury in a rat model. METHODS: Ten-millimeter segments of sciatic nerve were resected in 44 Lewis rats. The gaps were either left unrepaired (n = 6), repaired with nerve autograft (n = 19), or repaired with conduit (n = 19). After 12 weeks, nerve conduction latency, compound muscle action potential amplitude, muscle force and muscle mass were measured. The numbers of axons and axon diameters both within the grafts and distally were determined. RESULTS: After 12 weeks, gastrocnemius isometric tetanic force and muscle mass for the conduit group reached 85 and 82% of autograft values, respectively. Nerve conduction and compound muscle action potential were not significantly different between these two groups, although the latter approached significance. There was no recovery in the unrepaired group. CONCLUSION: Muscle recovery for the animals treated with this aligned nanofiber conduit approached that of autograft, suggesting the importance of internal conduit structure for nerve repair.
Authors: Jenny Jin; Sonja Limburg; Sunil K Joshi; Rebeccah Landman; Michelle Park; Qia Zhang; Hubert T Kim; Alfred C Kuo Journal: Tissue Eng Part A Date: 2013-06-15 Impact factor: 3.845
Authors: Andrew Li; Akishige Hokugo; Anisa Yalom; Eric J Berns; Nicholas Stephanopoulos; Mark T McClendon; Luis A Segovia; Igor Spigelman; Samuel I Stupp; Reza Jarrahy Journal: Biomaterials Date: 2014-07-23 Impact factor: 12.479
Authors: Arkadiusz Jundziłł; Marta Pokrywczyńska; Jan Adamowicz; Tomasz Kowalczyk; Maciej Nowacki; Magdalena Bodnar; Andrzej Marszałek; Małgorzata Frontczak-Baniewicz; Grzegorz Mikułowski; Tomasz Kloskowski; James Gatherwright; Tomasz Drewa Journal: Med Sci Monit Date: 2017-03-31