| Literature DB >> 23161424 |
Jui-Yu Wu1, Ming-Chih Chang, Chien-Shu Chen, Hsiu-Chen Lin, Hsiang-Ping Tsai, Chien-Chun Yang, Che-Hsiung Yang, Chun-Mao Lin.
Abstract
Topoisomerase inhibitors have been developed in a variety of clinical applications. We investigated the inhibitory effect of evodiamine on E. coli topoisomerase I, which may lead to an anti-bacterial effect. Evodiamine inhibits the supercoiled plasmid DNA relaxation that is catalyzed by E. coli topoisomerase I, and computer-aided docking has shown that the Arg161 and Asp551 residues of topoisomerase I interact with evodiamine. We investigated the bactericidal effect of evodiamine against multidrug-resistant Klebsiella pneumoniae. Evodiamine showed a significantly lower minimal inhibitory concentration value (MIC 128 µg/mL) compared with antibiotics (>512 µg/mL) against the clinical isolate of K. pneumoniae. The results suggested that evodiamine is a potential agent against drug-resistant bacteria. Georg Thieme Verlag KG Stuttgart · New York.Entities:
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Year: 2012 PMID： 23161424 DOI： 10.1055/s-0032-1327925
Source DB: PubMed Journal: Planta Med ISSN： 0032-0943 Impact factor: 3.352