| Literature DB >> 23158309 |
Elisabete Paula Barros1, Nathalie Moreira, Giuliano Elias Pereira, Selma Gomes Ferreira Leite, Claudia Moraes Rezende, Paula Guedes de Pinho.
Abstract
An automated headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-ion trap/mass spectrometry (GC-IT/MS) was developed in order to quantify a large number of volatile compounds in wines such as alcohols, ester, norisoprenoids and terpenes. The procedures were optimized for SPME fiber selection, pre-incubation temperature and time, extraction temperature and time, and salt addition. A central composite experimental design was used in the optimization of the extraction conditions. The volatile compounds showed optimal extraction using a DVB/CAR/PDMS fiber, incubation of 5 ml of wine with 2g NaCl at 45 °C during 5 min, and subsequent extraction of 30 min at the same temperature. The method allowed the identification of 64 volatile compounds. Afterwards, the method was validated successfully for the most significant compounds and was applied to study the volatile composition of different white wines.Entities:
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Year: 2012 PMID： 23158309 DOI： 10.1016/j.talanta.2012.08.028
Source DB: PubMed Journal: Talanta ISSN： 0039-9140 Impact factor: 6.057