Mariana Carvalho Andrade1, Ana Paula Dias Ribeiro2, Lívia Nordi Dovigo3, Iguatemy Lourenço Brunetti4, Eunice Teresinha Giampaolo1, Vanderlei Salvador Bagnato5, Ana Cláudia Pavarina6. 1. Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ Estadual Paulista, Araraquara, SP, Brazil. 2. Department of Operative Dentistry, University of Brasília - UnB, Brasília, DF, Brazil. 3. Department of Social Dentistry, Araraquara Dental School, UNESP - Univ Estadual Paulista, Araraquara, Araraquara, SP, Brazil. 4. Department of Clinical Analysis, UNESP - Univ Estadual Paulista, Araraquara, SP, Brazil. 5. Physics Institute of São Carlos, University of São Paulo - USP, São Carlos, SP, Brazil. 6. Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ Estadual Paulista, Araraquara, SP, Brazil. Electronic address: pavarina@foar.unesp.br.
Abstract
OBJECTIVES: The aim of this study was to evaluate the effects of pre-irradiation time (PIT) on curcumin (Cur)-mediated photodynamic therapy (PDT) against planktonic and biofilm cultures of reference strains of Candida albicans, Candida glabrata and Candida dubliniensis. MATERIALS AND METHODS: Suspensions and biofilms of Candida species were maintained in contact with different concentrations of Cur for time intervals of 1, 5, 10 and 20min before irradiation and LED (light emitting diode) activation. Additional samples were treated only with Cur, without illumination, or only with light, without Cur. Control samples received neither light nor Cur. After PDT, suspensions were plated on Sabouraud Dextrose Agar, while biofilm results were obtained using the XTT-salt reduction method. Confocal Laser Scanning Microscopy (CLSM) observations were performed to supply a better understanding of Cur penetration through the biofilms after 5 and 20min of contact with the cultures. RESULTS: Different PITs showed no statistical differences in Cur-mediated PDT of Candida spp. cell suspensions. There was complete inactivation of the three Candida species with the association of 20.0μM Cur after 5, 10 and 20min of PIT. Biofilm cultures showed significant reduction in cell viability after PDT. In general, the three Candida species evaluated in this study suffered higher reductions in cell viability with the association of 40.0μM Cur and 20min of PIT. Additionally, CLSM observations showed different intensities of fluorescence emissions after 5 and 20min of incubation. CONCLUSION: Photoinactivation of planktonic cultures was not PIT-dependent. PIT-dependence of the biofilm cultures differed among the species evaluated. Also, CLSM observations confirmed the need of higher time intervals for the Cur to penetrate biofilm structures.
OBJECTIVES: The aim of this study was to evaluate the effects of pre-irradiation time (PIT) on curcumin (Cur)-mediated photodynamic therapy (PDT) against planktonic and biofilm cultures of reference strains of Candida albicans, Candida glabrata and Candida dubliniensis. MATERIALS AND METHODS: Suspensions and biofilms of Candida species were maintained in contact with different concentrations of Cur for time intervals of 1, 5, 10 and 20min before irradiation and LED (light emitting diode) activation. Additional samples were treated only with Cur, without illumination, or only with light, without Cur. Control samples received neither light nor Cur. After PDT, suspensions were plated on Sabouraud Dextrose Agar, while biofilm results were obtained using the XTT-salt reduction method. Confocal Laser Scanning Microscopy (CLSM) observations were performed to supply a better understanding of Cur penetration through the biofilms after 5 and 20min of contact with the cultures. RESULTS: Different PITs showed no statistical differences in Cur-mediated PDT of Candida spp. cell suspensions. There was complete inactivation of the three Candida species with the association of 20.0μM Cur after 5, 10 and 20min of PIT. Biofilm cultures showed significant reduction in cell viability after PDT. In general, the three Candida species evaluated in this study suffered higher reductions in cell viability with the association of 40.0μM Cur and 20min of PIT. Additionally, CLSM observations showed different intensities of fluorescence emissions after 5 and 20min of incubation. CONCLUSION: Photoinactivation of planktonic cultures was not PIT-dependent. PIT-dependence of the biofilm cultures differed among the species evaluated. Also, CLSM observations confirmed the need of higher time intervals for the Cur to penetrate biofilm structures.
Authors: Juliana Guerra Pinto; Letícia Correa Fontana; Marco Antonio de Oliveira; Cristina Kurachi; Leandro José Raniero; Juliana Ferreira-Strixino Journal: Lasers Med Sci Date: 2016-04-07 Impact factor: 3.161
Authors: Paula Volpato Sanitá; Ana Cláudia Pavarina; Lívia Nordi Dovigo; Ana Paula Dias Ribeiro; Mariana Carvalho Andrade; Ewerton Garcia de Oliveira Mima Journal: Lasers Med Sci Date: 2017-11-13 Impact factor: 3.161
Authors: Francisco G Rego-Filho; Maria T de Araujo; Kleber T de Oliveira; Vanderlei S Bagnato Journal: J Fluoresc Date: 2014-07-03 Impact factor: 2.217
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