Literature DB >> 23150440

Identification of small molecules affecting p53-MDM2/MDMX interaction by fluorescence polarization.

Qi Zhang1, Hua Lu.   

Abstract

Fluorescence polarization (FP) has become a powerful technique to quantitatively analyze the binding of a small soluble fluorescence-labeled probe to a larger soluble protein and its displacement by other molecules. Here, we describe a detailed protocol to identify small molecules capable of targeting p53-MDM2/MDMX interactions using a fluorescence polarization assay with Rhodamine-labeled p53 peptides.

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Year:  2013        PMID： 23150440      PMCID： PMC3760520          DOI： 10.1007/978-1-62703-236-0_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

1.  High efficiency transformation of Escherichia coli with plasmids.

Authors:  H Inoue; H Nojima; H Okayama
Journal:  Gene       Date:  1990-11-30       Impact factor: 3.688

2.  Structure-based design of potent non-peptide MDM2 inhibitors.

Authors:  Ke Ding; Yipin Lu; Zaneta Nikolovska-Coleska; Su Qiu; Yousong Ding; Wei Gao; Jeanne Stuckey; Krzysztof Krajewski; Peter P Roller; York Tomita; Damon A Parrish; Jeffrey R Deschamps; Shaomeng Wang
Journal:  J Am Chem Soc       Date:  2005-07-27       Impact factor: 15.419

3.  Structure-based design of spiro-oxindoles as potent, specific small-molecule inhibitors of the MDM2-p53 interaction.

Authors:  Ke Ding; Yipin Lu; Zaneta Nikolovska-Coleska; Guoping Wang; Su Qiu; Sanjeev Shangary; Wei Gao; Dongguang Qin; Jeanne Stuckey; Krzysztof Krajewski; Peter P Roller; Shaomeng Wang
Journal:  J Med Chem       Date:  2006-06-15       Impact factor: 7.446

4.  High affinity interaction of the p53 peptide-analogue with human Mdm2 and Mdmx.

Authors:  Anna Czarna; Grzegorz M Popowicz; Aleksandra Pecak; Siglinde Wolf; Grzegorz Dubin; Tad A Holak
Journal:  Cell Cycle       Date:  2009-04-16       Impact factor: 4.534

5.  Structure-based design of high affinity peptides inhibiting the interaction of p53 with MDM2 and MDMX.

Authors:  Jason Phan; Zhenyu Li; Agnieszka Kasprzak; Baozong Li; Said Sebti; Wayne Guida; Ernst Schönbrunn; Jiandong Chen
Journal:  J Biol Chem       Date:  2009-11-12       Impact factor: 5.157

6.  Novel pyrrolopyrimidine-based α-helix mimetics: cell-permeable inhibitors of protein−protein interactions.

Authors:  Ji Hoon Lee; Qi Zhang; Sunhwan Jo; Sergio C Chai; Misook Oh; Wonpil Im; Hua Lu; Hyun-Suk Lim
Journal:  J Am Chem Soc       Date:  2011-02-02       Impact factor: 15.419

7.  Identification and characterization of the first small molecule inhibitor of MDMX.

Authors:  Damon Reed; Ying Shen; Anang A Shelat; Leggy A Arnold; Antonio M Ferreira; Fangyi Zhu; Nicholas Mills; David C Smithson; Catherine A Regni; Donald Bashford; Samantha A Cicero; Brenda A Schulman; Aart G Jochemsen; R Kiplin Guy; Michael A Dyer
Journal:  J Biol Chem       Date:  2010-01-15       Impact factor: 5.157

8.  Temporal activation of p53 by a specific MDM2 inhibitor is selectively toxic to tumors and leads to complete tumor growth inhibition.

Authors:  Sanjeev Shangary; Dongguang Qin; Donna McEachern; Meilan Liu; Rebecca S Miller; Su Qiu; Zaneta Nikolovska-Coleska; Ke Ding; Guoping Wang; Jianyong Chen; Denzil Bernard; Jian Zhang; Yipin Lu; Qingyang Gu; Rajal B Shah; Kenneth J Pienta; Xiaolan Ling; Sanmao Kang; Ming Guo; Yi Sun; Dajun Yang; Shaomeng Wang
Journal:  Proc Natl Acad Sci U S A       Date:  2008-03-03       Impact factor: 11.205

9.  Structural basis for high-affinity peptide inhibition of p53 interactions with MDM2 and MDMX.

Authors:  Marzena Pazgier; Min Liu; Guozhang Zou; Weirong Yuan; Changqing Li; Chong Li; Jing Li; Juahdi Monbo; Davide Zella; Sergey G Tarasov; Wuyuan Lu
Journal:  Proc Natl Acad Sci U S A       Date:  2009-03-02       Impact factor: 11.205
  9 in total
  3 in total

1.  Directed Evolution Using Stabilized Bacterial Peptide Display.

Authors:  Tejas Navaratna; Lydia Atangcho; Mukesh Mahajan; Vivekanandan Subramanian; Marshall Case; Andrew Min; Daniel Tresnak; Greg M Thurber
Journal:  J Am Chem Soc       Date:  2020-01-10       Impact factor: 15.419

2.  The novel BH3 α-helix mimetic JY-1-106 induces apoptosis in a subset of cancer cells (lung cancer, colon cancer and mesothelioma) by disrupting Bcl-xL and Mcl-1 protein-protein interactions with Bak.

Authors:  Xiaobo Cao; Jeremy L Yap; M Karen Newell-Rogers; Chander Peddaboina; Weihua Jiang; Harry T Papaconstantinou; Dan Jupitor; Arun Rai; Kwan-Young Jung; Richard P Tubin; Wenbo Yu; Kenno Vanommeslaeghe; Paul T Wilder; Alexander D MacKerell; Steven Fletcher; Roy W Smythe
Journal:  Mol Cancer       Date:  2013-05-16       Impact factor: 27.401

3.  Inhibition of Mdmx (Mdm4) in vivo induces anti-obesity effects.

Authors:  Ning Kon; Donglai Wang; Tongyuan Li; Le Jiang; Li Qiang; Wei Gu
Journal:  Oncotarget       Date:  2018-01-02
  3 in total

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