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Literature DB >> 23150380

Optimized technique for subretinal injections in mice.

Regine Mühlfriedel¹, Stylianos Michalakis, Marina Garcia Garrido, Martin Biel, Mathias W Seeliger.

Abstract

Subretinal injections in mice become increasingly important. Currently, the most prominent application is in gene therapy of inherited eye diseases by means of viral vector delivery to photoreceptors or the retinal pigment epithelium (RPE). Since there are no large animal models for most of these diseases, genetically modified mouse models are commonly used in preclinical proof-of-concept studies. However, because of the relatively small mouse eye, adverse effects of the subretinal delivery procedure itself may interfere with the therapeutic outcome. The protocol described here concerns a transscleral pars plana subretinal injection in small eyes, and may be used for but not limited to virus-mediated gene transfer.

Entities: Disease Species

Mesh：

Year: 2013 PMID： 23150380 DOI： 10.1007/978-1-62703-080-9_24

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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Cited

13 in total

1. An Alternative and Validated Injection Method for Accessing the Subretinal Space via a Transcleral Posterior Approach.

Authors: Sachin Parikh; Andrew Le; Julian Davenport; Michael B Gorin; Steven Nusinowitz; Anna Matynia
Journal: J Vis Exp Date: 2016-12-07 Impact factor: 1.355

Review 2. Subretinal injection in mice to study retinal physiology and disease.

Authors: Peirong Huang; Siddharth Narendran; Felipe Pereira; Shinichi Fukuda; Yosuke Nagasaka; Ivana Apicella; Praveen Yerramothu; Kenneth M Marion; Xiaoyu Cai; Srinivas R Sadda; Bradley D Gelfand; Jayakrishna Ambati
Journal: Nat Protoc Date: 2022-04-13 Impact factor: 13.491

3. Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research.

Authors: Mark C Butler; Jack M Sullivan
Journal: J Vis Exp Date: 2018-11-02 Impact factor: 1.355

4. Subretinal AAV2.COMP-Ang1 suppresses choroidal neovascularization and vascular endothelial growth factor in a murine model of age-related macular degeneration.

Authors: Nathan G Lambert; Xiaohui Zhang; Ruju R Rai; Hironori Uehara; Susie Choi; Lara S Carroll; Subrata K Das; Judd M Cahoon; Brian H Kirk; Blaine M Bentley; Balamurali K Ambati
Journal: Exp Eye Res Date: 2016-01-13 Impact factor: 3.467

5. AAV-Mediated Gene Supplementation Therapy in Achromatopsia Type 2: Preclinical Data on Therapeutic Time Window and Long-Term Effects.

Authors: Regine Mühlfriedel; Naoyuki Tanimoto; Christian Schön; Vithiyanjali Sothilingam; Marina Garcia Garrido; Susanne C Beck; Gesine Huber; Martin Biel; Mathias W Seeliger; Stylianos Michalakis
Journal: Front Neurosci Date: 2017-05-24 Impact factor: 4.677

Review 6. Pluripotent Stem Cells for Retinal Tissue Engineering: Current Status and Future Prospects.

Authors: Ratnesh Singh; Oscar Cuzzani; François Binette; Hal Sternberg; Michael D West; Igor O Nasonkin
Journal: Stem Cell Rev Rep Date: 2018-08 Impact factor: 5.739

7. A universal protocol for isolating retinal ON bipolar cells across species via fluorescence-activated cell sorting.

Authors: Elisa Murenu; Marina Pavlou; Lisa Richter; Kleopatra Rapti; Sabrina Just; Jasmina Cehajic-Kapetanovic; Neda Tafrishi; Andrew Hayes; Rachel Scholey; Robert Lucas; Hildegard Büning; Dirk Grimm; Stylianos Michalakis
Journal: Mol Ther Methods Clin Dev Date: 2021-01-26 Impact factor: 6.698