Literature DB >> 23144001

Real-time fluorescence turn-on detection of alkaline phosphatase activity with a novel perylene probe.

Jian Chen1, Huping Jiao, Wenying Li, Dongli Liao, Huipeng Zhou, Cong Yu.   

Abstract

A tetracationic perylene probe (probe 1) was designed and synthesized. Probe 1 was used for the real-time fluorescence turn-on assay of alkaline phosphatase (ALP) activity and inhibitor screening. Probe 1 monomer fluorescence could be very efficiently quenched by ATP through the formation of an ATP/probe 1 complex. ALP triggered the degradation of ATP, the breakdown of the ATP/probe 1 complex, and the recovery of the probe 1 monomer fluorescence. In the presence of an ALP inhibitor, a decrease in fluorescence recovery was observed.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Year:  2012        PMID： 23144001     DOI： 10.1002/asia.201200880

Source DB:  PubMed          Journal:  Chem Asian J        ISSN： 1861-471X


  3 in total

1.  Controlled synthesis of polydopamine: A new strategy for highly sensitive fluorescence turn-on detection of acetylcholinesterase activity.

Authors:  Meiding Yang; Huipeng Zhou; Yunyi Zhang; Zhenzhen Hu; Niu Niu; Cong Yu
Journal:  Mikrochim Acta       Date:  2018-01-25       Impact factor: 5.833

2.  Inner filter effect based fluorometric determination of the activity of alkaline phosphatase by using carbon dots codoped with boron and nitrogen.

Authors:  Mi Mao; Tian Tian; Yu He; Yili Ge; Jiangang Zhou; Gongwu Song
Journal:  Mikrochim Acta       Date:  2017-12-05       Impact factor: 5.833

3.  Fluorometric determination of the activity of alkaline phosphatase based on the competitive binding of gold nanoparticles and pyrophosphate to CePO4:Tb nanorods.

Authors:  Ai-Zhen Xu; Li Zhang; Hui-Hui Zeng; Ru-Ping Liang; Jian-Ding Qiu
Journal:  Mikrochim Acta       Date:  2018-05-09       Impact factor: 5.833
  3 in total

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