Literature DB >> 23142659

Estimated copy number of Bacillus anthracis plasmids pXO1 and pXO2 using digital PCR.

Timothy Straub1, Cheryl Baird, Rachel A Bartholomew, Heather Colburn, Derrick Seiner, Kristin Victry, Li Zhang, Cynthia J Bruckner-Lea.   

Abstract

We evaluated digital PCR (dPCR) to directly enumerate plasmid and chromosome copies in three strains of Bacillus anthracis. Copy number estimates based on conventional quantitative PCR (qPCR) highlighted the variability of using qPCR to measure copy number whereas estimates based on direct sequencing are comparable to dPCR.
Copyright © 2012 Elsevier B.V. All rights reserved.

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Year:  2012        PMID： 23142659     DOI： 10.1016/j.mimet.2012.10.013

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  11 in total

1.  Identification of three noncontiguous regions on Bacillus anthracis plasmid pXO1 that are important for its maintenance.

Authors:  Andrei P Pomerantsev; Zanetta Chang; Catherine Rappole; Stephen H Leppla
Journal:  J Bacteriol       Date:  2014-06-09       Impact factor: 3.490

2.  Three-color crystal digital PCR.

Authors:  J Madic; A Zocevic; V Senlis; E Fradet; B Andre; S Muller; R Dangla; M E Droniou
Journal:  Biomol Detect Quantif       Date:  2016-11-03

3.  Quantitative PCR and Digital PCR for Detection of Ascaris lumbricoides Eggs in Reclaimed Water.

Authors:  Lucrecia Acosta Soto; Ana Belén Santísima-Trinidad; Fernando Jorge Bornay-Llinares; Marcos Martín González; José Antonio Pascual Valero; Margarita Ros Muñoz
Journal:  Biomed Res Int       Date:  2017-03-09       Impact factor: 3.411

4.  Genomic Characterization and Copy Number Variation of Bacillus anthracis Plasmids pXO1 and pXO2 in a Historical Collection of 412 Strains.

Authors:  Angela Pena-Gonzalez; Luis M Rodriguez-R; Chung K Marston; Jay E Gee; Christopher A Gulvik; Cari B Kolton; Elke Saile; Michael Frace; Alex R Hoffmaster; Konstantinos T Konstantinidis
Journal:  mSystems       Date:  2018-08-14       Impact factor: 6.496

5.  Ultrasensitive Detection of Bacillus anthracis by Real-Time PCR Targeting a Polymorphism in Multi-Copy 16S rRNA Genes and Their Transcripts.

Authors:  Peter Braun; Martin Duy-Thanh Nguyen; Mathias C Walter; Gregor Grass
Journal:  Int J Mol Sci       Date:  2021-11-12       Impact factor: 5.923

6.  Microevolution of Anthrax from a Young Ancestor (M.A.Y.A.) Suggests a Soil-Borne Life Cycle of Bacillus anthracis.

Authors:  Peter Braun; Gregor Grass; Angela Aceti; Luigina Serrecchia; Alessia Affuso; Leonardo Marino; Stefania Grimaldi; Stefania Pagano; Matthias Hanczaruk; Enrico Georgi; Bernd Northoff; Anne Schöler; Michael Schloter; Markus Antwerpen; Antonio Fasanella
Journal:  PLoS One       Date:  2015-08-12       Impact factor: 3.240

7.  DNA Persistence in a Sink Drain Environment.

Authors:  Eric M Winder; George T Bonheyo
Journal:  PLoS One       Date:  2015-07-31       Impact factor: 3.240

8.  Avirulent Bacillus anthracis Strain with Molecular Assay Targets as Surrogate for Irradiation-Inactivated Virulent Spores.

Authors:  Roger D Plaut; Andrea B Staab; Mark A Munson; Joan S Gebhardt; Christopher P Klimko; Avery V Quirk; Christopher K Cote; Tony L Buhr; Rebecca D Rossmaier; Robert C Bernhards; Courtney E Love; Kimberly L Berk; Teresa G Abshire; David A Rozak; Linda C Beck; Scott Stibitz; Bruce G Goodwin; Michael A Smith; Shanmuga Sozhamannan
Journal:  Emerg Infect Dis       Date:  2018-04       Impact factor: 6.883

9.  High-Quality Genome Sequence of Bacillus anthracis Strain 14RA5914 Isolated during an Outbreak in Germany in 2014.

Authors:  Mandy C Elschner; Anne Busch; Annette Schliephake; Wolfgang Gaede; Eric Zuchantke; Herbert Tomaso
Journal:  Genome Announc       Date:  2017-10-05

10.  Duplex Electrochemical DNA Sensor to Detect Bacillus anthracis CAP and PAG DNA Targets Based on the Incorporation of Tailed Primers and Ferrocene-Labeled dATP.

Authors:  Ivan Magriñá; Miriam Jauset-Rubio; Mayreli Ortiz; Herbert Tomaso; Anna Simonova; Michal Hocek; Ciara K O'Sullivan
Journal:  ACS Omega       Date:  2019-12-11
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