Determination of melamine by flow injection analysis based on chemiluminescence system.

In this paper, based upon the phenomenon that melamine can obviously enhance the CL signal of the luminol-H(2)O(2) system in basic medium, a simple, rapid and sensitive flow injection chemiluminescence (FI-CL) method for the determination of melamine has been developed. Under the optimum conditions, the linear range for the determination of melamine was 0.2-80μgmL(-1) with a detection limit of 0.12μgmL(-1) calculated as proposed by IUPAC and a relative standard deviation of 3.26% for 11 solutions of 10μgmL(-1) melamine on the same day. The proposed method was satisfactorily applied to determine melamine in milk-based products and satisfactory results were obtained without interferences from the sample matrix. Moreover, one assay produce takes only 25s and the minimum sampling rate is about 120 samplesh(-1), which indicated that the FI-CL method was suitable for high throughput and real-time melamine analysis.