A rapid liquid chromatography method for determination of glufosinate residue in maize after derivatisation.

A rapid liquid chromatographic method for glufosinate analysis in maize samples after derivatisation has been developed. The labelled glufosinate was separated on a Kromasil C(18) column (250mm×4.6mm, 5μm) and UV detection was applied at 360nm. The optimisation of derivatisation conditions and the influence of different ion-pair reagents on the separation were discussed. The method linearity correlation coefficient was 0.9998 in concentrations ranging from 0.1 to 20mgL(-1). The level of quantification was set to 0.02mgkg(-1), and reached pesticide EU-MRLs for glufosinate in the maize samples. The proposed method was applied to the quantitative determination of glufosinate in samples with recoveries of 98.0-100.5% and RSDs of 2.13-4.13%.