Li-Li Cheng1, Cong-Ying Wu, Ming-He Sui. 1. School of Acu-moxibustion and Massage, Beijing University of Chinese Medicine, Beijing 100029, China. 2008beijingfuture@163.com
Abstract
OBJECTIVE: To observe the effect of electroacupuncture (EA) of "Weizhong" (BL 40), "Sanyinjiao" (SP 6) and "Yinlingquan" (SP 9) on changes of intravesical pressure and contents of adenosine triphosphate (ATP) in the urinary bladder tissue in rabbits with acute urine retention, so as to explore the relatively specificity of acupoints for urinary bladder problems. METHODS: Forty-eight male adult rabbits were randomly divided into normal control, model, Weizhong (BL 40, EA-BL 40), San-yinjiao (SP 6, EA-SP 6), Yinlingquan (SP9, EA-SP9) and non-acupoint (EA-NA) groups. Acute urinary retention model was established by filling the rabbits' bladder with normal saline at a volume of 2 times above their normal capacity for 2 hours. EA (2 Hz/15 Hz, 0.6 mA) was applied to bilateral "Weizhong" (BL 40), "Sanyinjiao" (SP 6) and "Yinlingquan" (SP 9) for 20 min, respectively. Intravesical pressure was detected by using a pressure transducer and an amplifier. Bladder ATP content was detected by using enzyme linked immunosorbent assay (ELISA). RESULTS: In comparison with pre-modeling, the intravesical pressure levels were decreased significantly after modeling in the model, EA-BL 40, EA-SP 6, EA-SP 9 and EA-NA groups (P < 0.05). Compared with pre-EA, intravesical pressure values were increased remarkably in the EA-BL 40, EA-SP 6, EA-SP 9 and EA-NA groups (P < 0.05). The percentages of the increased vesical pressure after EA were considerably higher in the EA-BL 40, EA-SP 6, EA-SP 9 and EA-NA groups than in the model group (P < 0.05). Compared with the normal group, bladder ATP content in the model group was reduced significantly (P < 0.05); while in comparison with the model group, ATP contents in the EA-BL 40, EA-SP 6 and EA-SP 9 groups were up-regulated apparently (P < 0.05). The bladder ATP level of the EA-BL 40 group was significantly higher than those of EA-SP 6 and EA-SP 9 groups (P < 0.05). No significant differences were found between the model and EA-NA groups, and between the EA-SP6 and EA-SP9 groups in bladder ATP contents (P > 0.05), and among the EA-BL 40, EA-SP 6 and EA-SP 9 groups in the percentages of the increased intravesical pressure (P > 0.05). CONCLUSION: EA of BL 40, SP 6 and SP 9 can significantly raise intravesical pressure and bladder ATP content in urine retention rabbits, which may contribute to its effect in improving urinary retention.
OBJECTIVE: To observe the effect of electroacupuncture (EA) of "Weizhong" (BL 40), "Sanyinjiao" (SP 6) and "Yinlingquan" (SP 9) on changes of intravesical pressure and contents of adenosine triphosphate (ATP) in the urinary bladder tissue in rabbits with acute urine retention, so as to explore the relatively specificity of acupoints for urinary bladder problems. METHODS: Forty-eight male adult rabbits were randomly divided into normal control, model, Weizhong (BL 40, EA-BL 40), San-yinjiao (SP 6, EA-SP 6), Yinlingquan (SP9, EA-SP9) and non-acupoint (EA-NA) groups. Acute urinary retention model was established by filling the rabbits' bladder with normal saline at a volume of 2 times above their normal capacity for 2 hours. EA (2 Hz/15 Hz, 0.6 mA) was applied to bilateral "Weizhong" (BL 40), "Sanyinjiao" (SP 6) and "Yinlingquan" (SP 9) for 20 min, respectively. Intravesical pressure was detected by using a pressure transducer and an amplifier. Bladder ATP content was detected by using enzyme linked immunosorbent assay (ELISA). RESULTS: In comparison with pre-modeling, the intravesical pressure levels were decreased significantly after modeling in the model, EA-BL 40, EA-SP 6, EA-SP 9 and EA-NA groups (P < 0.05). Compared with pre-EA, intravesical pressure values were increased remarkably in the EA-BL 40, EA-SP 6, EA-SP 9 and EA-NA groups (P < 0.05). The percentages of the increased vesical pressure after EA were considerably higher in the EA-BL 40, EA-SP 6, EA-SP 9 and EA-NA groups than in the model group (P < 0.05). Compared with the normal group, bladder ATP content in the model group was reduced significantly (P < 0.05); while in comparison with the model group, ATP contents in the EA-BL 40, EA-SP 6 and EA-SP 9 groups were up-regulated apparently (P < 0.05). The bladder ATP level of the EA-BL 40 group was significantly higher than those of EA-SP 6 and EA-SP 9 groups (P < 0.05). No significant differences were found between the model and EA-NA groups, and between the EA-SP6 and EA-SP9 groups in bladder ATP contents (P > 0.05), and among the EA-BL 40, EA-SP 6 and EA-SP 9 groups in the percentages of the increased intravesical pressure (P > 0.05). CONCLUSION: EA of BL 40, SP 6 and SP 9 can significantly raise intravesical pressure and bladder ATP content in urine retention rabbits, which may contribute to its effect in improving urinary retention.