OBJECTIVE: Bone Marrow Stem Cells (BMSC) are a 'reservoir' for bone regeneration. BMSC can be studied in vitro by cloning cells which are improperly named colonyforming units of fibroblasts (CFU-f). Thus we decided to study CFU-f organization and morphology to have (A) a parameter by which to compare normal and pathologic conditions and (B) to potentially select the most osteogenic clones. METHODS: Two hundred and forty bone samples were collected from 109 patients and primary cultures performed. RESULTS: After two weeks 9 cell types and 6 well organized types of colonies were detectable. Some have alkaline phosphatase (AP) activity. CONCLUSION: These data could be relevant to estimate the potential regeneration of bone.
OBJECTIVE: Bone Marrow Stem Cells (BMSC) are a 'reservoir' for bone regeneration. BMSC can be studied in vitro by cloning cells which are improperly named colonyforming units of fibroblasts (CFU-f). Thus we decided to study CFU-f organization and morphology to have (A) a parameter by which to compare normal and pathologic conditions and (B) to potentially select the most osteogenic clones. METHODS: Two hundred and forty bone samples were collected from 109 patients and primary cultures performed. RESULTS: After two weeks 9 cell types and 6 well organized types of colonies were detectable. Some have alkaline phosphatase (AP) activity. CONCLUSION: These data could be relevant to estimate the potential regeneration of bone.
Entities:
Keywords:
Bone production in vitro system; Clone; Morphology; Stem cell
Authors: Wolfgang Wagner; Rainer Saffrich; Ute Wirkner; Volker Eckstein; Jonathon Blake; Alexandra Ansorge; Christian Schwager; Frederik Wein; Katrin Miesala; Wilhelm Ansorge; Anthony D Ho Journal: Stem Cells Date: 2005-06-13 Impact factor: 6.277
Authors: Nina J Unger; Kathrin I Odörfer; Karin Weber; Eric P Sandgren; Reinhold G Erben Journal: Histochem Cell Biol Date: 2007-05-11 Impact factor: 4.304