Literature DB >> 23138948

Making cRNA for microinjection and expression of fluorescently tagged proteins for live-cell imaging in oocytes.

Mark Levasseur1.   

Abstract

Fluorescently tagged proteins have become a crucial weapon in the armory of a successful cell biology laboratory. This chapter describes how to produce cRNA coding for a fluorescently tagged protein of choice, such that it is suitable for microinjection and subsequent expression studies in live oocytes.

Mesh：

Substances：

Year:  2013        PMID： 23138948     DOI： 10.1007/978-1-62703-191-2_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  3 in total

1.  Aneuploidy in Oocytes Is Prevented by Sustained CDK1 Activity through Degron Masking in Cyclin B1.

Authors:  Mark D Levasseur; Christopher Thomas; Owen R Davies; Jonathan M G Higgins; Suzanne Madgwick
Journal:  Dev Cell       Date:  2019-02-07       Impact factor: 12.270

2.  A prometaphase mechanism of securin destruction is essential for meiotic progression in mouse oocytes.

Authors:  Christopher Thomas; Benjamin Wetherall; Mark D Levasseur; Rebecca J Harris; Scott T Kerridge; Jonathan M G Higgins; Owen R Davies; Suzanne Madgwick
Journal:  Nat Commun       Date:  2021-07-14       Impact factor: 14.919

3.  Chromosome biorientation and APC activity remain uncoupled in oocytes with reduced volume.

Authors:  Simon I R Lane; Keith T Jones
Journal:  J Cell Biol       Date:  2017-10-04       Impact factor: 10.539
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.