Literature DB >> 23135949

Clinical application of a multiplex real-time PCR assay for simultaneous detection of Legionella species, Legionella pneumophila, and Legionella pneumophila serogroup 1.

Alvaro J Benitez1, Jonas M Winchell.   

Abstract

We developed a single-tube multiplex real-time PCR assay capable of simultaneously detecting and discriminating Legionella spp., Legionella pneumophila, and Legionella pneumophila serogroup 1 in primary specimens. Evaluation of 21 clinical specimens and 115 clinical isolates demonstrated this assay to be a rapid, high-throughput diagnostic test with 100% specificity that may aid during legionellosis outbreaks and epidemiologic investigations.

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Year:  2012        PMID： 23135949      PMCID： PMC3536254          DOI： 10.1128/JCM.02510-12

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  Addition of neuA, the gene encoding N-acylneuraminate cytidylyl transferase, increases the discriminatory ability of the consensus sequence-based scheme for typing Legionella pneumophila serogroup 1 strains.

Authors:  Sandra Ratzow; Valeria Gaia; Jürgen Herbert Helbig; Norman K Fry; Paul Christian Lück
Journal:  J Clin Microbiol       Date:  2007-04-04       Impact factor: 5.948

2.  Identification of mip-like genes in the genus Legionella.

Authors:  N P Cianciotto; J M Bangsborg; B I Eisenstein; N C Engleberg
Journal:  Infect Immun       Date:  1990-09       Impact factor: 3.441

3.  Sequence-based classification scheme for the genus Legionella targeting the mip gene.

Authors:  R M Ratcliff; J A Lanser; P A Manning; M W Heuzenroeder
Journal:  J Clin Microbiol       Date:  1998-06       Impact factor: 5.948

Review 4.  The molecular ecology of legionellae.

Authors:  B S Fields
Journal:  Trends Microbiol       Date:  1996-07       Impact factor: 17.079

Review 5.  Legionella and Legionnaires' disease: 25 years of investigation.

Authors:  Barry S Fields; Robert F Benson; Richard E Besser
Journal:  Clin Microbiol Rev       Date:  2002-07       Impact factor: 26.132

6.  Infection due to Legionella species other than L. pneumophila.

Authors:  Robert R Muder; Victor L Yu
Journal:  Clin Infect Dis       Date:  2002-09-24       Impact factor: 9.079

7.  Distribution of Legionella species and serogroups isolated by culture in patients with sporadic community-acquired legionellosis: an international collaborative survey.

Authors:  Victor L Yu; Joseph F Plouffe; Maddalena Castellani Pastoris; Janet E Stout; Mona Schousboe; Andreas Widmer; James Summersgill; Thomas File; Christopher M Heath; David L Paterson; Annette Chereshsky
Journal:  J Infect Dis       Date:  2002-05-21       Impact factor: 5.226

8.  Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of Legionella pneumophila and other Legionella species.

Authors:  Kate E Templeton; Sitha A Scheltinga; Peter Sillekens; Jantine W Crielaard; Alje P van Dam; Herman Goossens; Eric C J Claas
Journal:  J Clin Microbiol       Date:  2003-09       Impact factor: 5.948

Review 9.  Legionella pneumophila: an aquatic microbe goes astray.

Authors:  Michael Steinert; Ute Hentschel; Jörg Hacker
Journal:  FEMS Microbiol Rev       Date:  2002-06       Impact factor: 16.408

10.  Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus.

Authors:  Shannon L Emery; Dean D Erdman; Michael D Bowen; Bruce R Newton; Jonas M Winchell; Richard F Meyer; Suxiang Tong; Byron T Cook; Brian P Holloway; Karen A McCaustland; Paul A Rota; Bettina Bankamp; Luis E Lowe; Tom G Ksiazek; William J Bellini; Larry J Anderson
Journal:  Emerg Infect Dis       Date:  2004-02       Impact factor: 6.883
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  22 in total

Review 1.  Atypical Pneumonia: Updates on Legionella, Chlamydophila, and Mycoplasma Pneumonia.

Authors:  Lokesh Sharma; Ashley Losier; Thomas Tolbert; Charles S Dela Cruz; Chad R Marion
Journal:  Clin Chest Med       Date:  2016-12-24       Impact factor: 2.878

Review 2.  Current and emerging Legionella diagnostics for laboratory and outbreak investigations.

Authors:  Jeffrey W Mercante; Jonas M Winchell
Journal:  Clin Microbiol Rev       Date:  2015-01       Impact factor: 26.132

3.  Legionnaires' disease pseudoepidemic due to falsely positive urine antigen test results.

Authors:  Maria Cristina Rota; Stefano Fontana; Carmen Montaño-Remacha; Maria Scaturro; Maria Grazia Caporali; Vincenzo Vullo; Laura Scorzolini; Andreina Ercole; Maria Luisa Ricci
Journal:  J Clin Microbiol       Date:  2014-04-09       Impact factor: 5.948

4.  Legionellosis and Recent Advances in Technologies for Legionella Control in Premise Plumbing Systems: A Review.

Authors:  Kelsie M Carlson; Laura A Boczek; Soryong Chae; Hodon Ryu
Journal:  Water (Basel)       Date:  2020-03-02       Impact factor: 3.103

Review 5.  Diagnostic Accuracy of PCR Alone and Compared to Urinary Antigen Testing for Detection of Legionella spp.: a Systematic Review.

Authors:  Tomer Avni; Amir Bieber; Hefziba Green; Tali Steinmetz; Leonard Leibovici; Mical Paul
Journal:  J Clin Microbiol       Date:  2015-12-09       Impact factor: 5.948

6.  Evaluation of Commercial Molecular Diagnostic Methods for Detection and Determination of Macrolide Resistance in Mycoplasma pneumoniae.

Authors:  Sixto M Leal; Arthur H Totten; Li Xiao; Donna M Crabb; Amy Ratliff; Lynn B Duffy; Karen B Fowler; Emily Mixon; Jonas M Winchell; Maureen H Diaz; Alvaro J Benitez; Bernard J Wolff; Xuan Qin; Y-W Tang; Mark Gonzalez; Raj Selvarangan; Tao Hong; Edward Brooks; Steve Dallas; T Prescott Atkinson; Xiaotian Zheng; Jennifer Dien Bard; Ken B Waites
Journal:  J Clin Microbiol       Date:  2020-05-26       Impact factor: 5.948

7.  Rapid detection and typing of pathogenic nonpneumophila Legionella spp. isolates using a multiplex real-time PCR assay.

Authors:  Alvaro J Benitez; Jonas M Winchell
Journal:  Diagn Microbiol Infect Dis       Date:  2016-01-12       Impact factor: 2.803

8.  Comparative genome analysis reveals a complex population structure of Legionella pneumophila subspecies.

Authors:  Natalia A Kozak-Muiznieks; Shatavia S Morrison; Jeffrey W Mercante; Maliha K Ishaq; Taccara Johnson; Jason Caravas; Claressa E Lucas; Ellen Brown; Brian H Raphael; Jonas M Winchell
Journal:  Infect Genet Evol       Date:  2018-02-07       Impact factor: 4.393

9.  Simultaneous detection of Legionella species and L. anisa, L. bozemanii, L. longbeachae and L. micdadei using conserved primers and multiple probes in a multiplex real-time PCR assay.

Authors:  Kristen E Cross; Jeffrey W Mercante; Alvaro J Benitez; Ellen W Brown; Maureen H Diaz; Jonas M Winchell
Journal:  Diagn Microbiol Infect Dis       Date:  2016-04-02       Impact factor: 2.803

Review 10.  Diagnostic testing for Legionnaires' disease.

Authors:  David M Pierre; Julianne Baron; Victor L Yu; Janet E Stout
Journal:  Ann Clin Microbiol Antimicrob       Date:  2017-08-29       Impact factor: 3.944
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