Literature DB >> 23132567

Construction of an artificial pathway for isobutanol biosynthesis in the cytosol of Saccharomyces cerevisiae.

Fumio Matsuda1, Takashi Kondo, Kengo Ida, Hironori Tezuka, Jun Ishii, Akihiko Kondo.   

Abstract

To increase isobutanol production in Saccharomyces cerevisiae, the valine biosynthetic pathway was activated by overexpression of the relevant enzymes in the mitochondria and the cytosol. Native mitochondrial enzymes were overepxressed in the cytosol by deleting the mitochondrial transit peptides. The metabolically engineered S. cerevisiae possessing the cytosolic pathway showed increased isobutanol production (63 ± 4 mg/L).

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Year:  2012        PMID： 23132567     DOI： 10.1271/bbb.120420

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  6 in total

1.  Production of aroma compounds from whey using Wickerhamomyces pijperi.

Authors:  Naoki Izawa; Miyuki Kudo; Yukako Nakamura; Harumi Mizukoshi; Takahiro Kitada; Toshiro Sone
Journal:  AMB Express       Date:  2015-04-16       Impact factor: 3.298

2.  Eliminating the isoleucine biosynthetic pathway to reduce competitive carbon outflow during isobutanol production by Saccharomyces cerevisiae.

Authors:  Kengo Ida; Jun Ishii; Fumio Matsuda; Takashi Kondo; Akihiko Kondo
Journal:  Microb Cell Fact       Date:  2015-04-29       Impact factor: 5.328

3.  A pyruvate carbon flux tugging strategy for increasing 2,3-butanediol production and reducing ethanol subgeneration in the yeast Saccharomyces cerevisiae.

Authors:  Jun Ishii; Keisuke Morita; Kengo Ida; Hiroko Kato; Shohei Kinoshita; Shoko Hataya; Hiroshi Shimizu; Akihiko Kondo; Fumio Matsuda
Journal:  Biotechnol Biofuels       Date:  2018-06-26       Impact factor: 6.040

4.  Biosensor for branched-chain amino acid metabolism in yeast and applications in isobutanol and isopentanol production.

Authors:  Jeremy D Cortez; Sarah K Hammer; Yanfei Zhang; César Carrasco-López; Sergio Á García Echauri; Jessica B Wiggins; Wei Wang; José L Avalos
Journal:  Nat Commun       Date:  2022-01-12       Impact factor: 14.919

5.  Microbial engineering for the production of isobutanol: current status and future directions.

Authors:  Nair M Lakshmi; Parameswaran Binod; Raveendran Sindhu; Mukesh Kumar Awasthi; Ashok Pandey
Journal:  Bioengineered       Date:  2021-12       Impact factor: 3.269

6.  Increased isobutanol production in Saccharomyces cerevisiae by eliminating competing pathways and resolving cofactor imbalance.

Authors:  Fumio Matsuda; Jun Ishii; Takashi Kondo; Kengo Ida; Hironori Tezuka; Akihiko Kondo
Journal:  Microb Cell Fact       Date:  2013-12-05       Impact factor: 5.328
  6 in total

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