Literature DB >> 2312349

Effect of tulipin on cell cycle progression analyzed by BrdUrd incorporation.

M Vitale1, S Papa, L Roncuzzi, A Gasperi-Campani.   

Abstract

The effect of tulipin, a protein from plant origin recently purified, on cell cycle progression has been analyzed in the sensitive EUE cells by BrdUrd incorporation. The cytofluorimetric results demonstrate that tulipin specifically interacts with the S phase, with a dose-dependent decrease of the total S phase cells and an increase of the G1/G2 cells after 4 h of treatment in the synchronized EUE cells, whereas in the asynchronous population it mainly causes a dose-dependent decrease in the incorporation of BrdUrd per cell.

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Year:  1990        PMID： 2312349     DOI： 10.1007/bf00266382

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  6 in total

1.  Purification, characterization and biological activity of tulipin, a novel inhibitor of DNA synthesis of plant origin.

Authors:  A Gasperi-Campani; E Lorenzoni; A Abbondanza; P Perocco; A I Falasca
Journal:  Anticancer Res       Date:  1987 Mar-Apr       Impact factor: 2.480

2.  In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain.

Authors:  T R Chen
Journal:  Exp Cell Res       Date:  1977-02       Impact factor: 3.905

3.  On the distribution of ribosome-inactivating proteins amongst plants.

Authors:  A Gasperi-Campani; L Barbieri; M G Battelli; F Stirpe
Journal:  J Nat Prod       Date:  1985 May-Jun       Impact factor: 4.050

4.  Differential activity of saporin 6 on normal and leukemic hemopoietic cells.

Authors:  A Gasperi-Campani; G Zauli; L Roncuzzi; L Valvassori; L Vitale; L Gaggioli; G P Bagnara
Journal:  Exp Hematol       Date:  1989-08       Impact factor: 3.084

5.  Gradient fractionation of cycling and resting cells monitored by BrdUrd incorporation.

Authors:  S Papa; M Vitale; G Mazzotti; R Rizzoli; M Falconi; A Bartoletti; F A Manzoli
Journal:  Histochemistry       Date:  1988

6.  Synchronization of HeLa cell cultures by inhibition of DNA polymerase alpha with aphidicolin.

Authors:  G Pedrali-Noy; S Spadari; A Miller-Faurès; A O Miller; J Kruppa; G Koch
Journal:  Nucleic Acids Res       Date:  1980-01-25       Impact factor: 16.971
  6 in total

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