Salinicola zeshunii sp. nov., a moderately halophilic bacterium isolated from soil of a chicken farm.

The taxonomic status of a moderately halophilic bacterium, strain N4(T), isolated from soil of a chicken farm in China was determined. It was Gram-negative, non-spore-forming, motile, and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequence indicated that this strain belonged to the genus Salinicola, as it showed the highest sequence similarities to Salinicola salaries M27(T) (98.3 %), Salinicola socius SMB35(T) (98.1 %), and Salinicola halophilus CG4.1(T) (98.1 %). The major cellular fatty acids were C(16:0) (25.6 %), C(18:1)ω7c (35.0 %), and C(19:0) cyclo ω8c (11.9 %), which are properties shared by members of the genus Salinicola. The DNA G+C content of strain N4(T) was 69.1 mol %. The level of DNA-DNA relatedness between strain N4(T) and the other three type strains of the genus of Salinicola salaries M27(T), Salinicola socius SMB35(T), and Salinicola halophilus CG4.1(T) were 34.3, 28.7, and 26.9 %, respectively. Based on the results of phenotypic, chemotaxonomic, DNA-DNA relatedness, and phylogenetic analysis, strain N4(T) should be classified as a novel species of the genus Salinicola, for which the name Salinicola zeshunii sp. nov. is proposed, with strain N4(T) (=KACC 16602(T) = CCTCC AB 2012912(T)) as the type strain.