Literature DB >> 23115292

A hepatitis C virus NS5A phosphorylation site that regulates RNA replication.

K L Lemay1, J Treadaway, I Angulo, T L Tellinghuisen.   

Abstract

The hepatitis C virus NS5A protein is essential for RNA replication and virion assembly. NS5A is phosphorylated on multiple residues during infections, but these sites remain uncharacterized. Here we identify serine 222 of genotype 2a NS5A as a phosphorylation site that functions as a negative regulator of RNA replication. This site is a component of the hyperphosphorylated form of NS5A, which is in good agreement with previous observations that hyperphosphorylation negatively affects replication.

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Year:  2012        PMID： 23115292      PMCID： PMC3554050          DOI： 10.1128/JVI.02154-12

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  28 in total

1.  Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase.

Authors:  T R Fuerst; E G Niles; F W Studier; B Moss
Journal:  Proc Natl Acad Sci U S A       Date:  1986-11       Impact factor: 11.205

2.  Production of infectious hepatitis C virus in tissue culture from a cloned viral genome.

Authors:  Takaji Wakita; Thomas Pietschmann; Takanobu Kato; Tomoko Date; Michiko Miyamoto; Zijiang Zhao; Krishna Murthy; Anja Habermann; Hans-Georg Kräusslich; Masashi Mizokami; Ralf Bartenschlager; T Jake Liang
Journal:  Nat Med       Date:  2005-06-12       Impact factor: 53.440

3.  Construction and characterization of infectious intragenotypic and intergenotypic hepatitis C virus chimeras.

Authors:  Thomas Pietschmann; Artur Kaul; George Koutsoudakis; Anna Shavinskaya; Stephanie Kallis; Eike Steinmann; Karim Abid; Francesco Negro; Marlene Dreux; Francois-Loic Cosset; Ralf Bartenschlager
Journal:  Proc Natl Acad Sci U S A       Date:  2006-05-01       Impact factor: 11.205

4.  The hepatitis C virus NS4B protein can trans-complement viral RNA replication and modulates production of infectious virus.

Authors:  Daniel M Jones; Arvind H Patel; Paul Targett-Adams; John McLauchlan
Journal:  J Virol       Date:  2008-12-10       Impact factor: 5.103

5.  The NS5A protein of hepatitis C virus is a zinc metalloprotein.

Authors:  Timothy L Tellinghuisen; Joseph Marcotrigiano; Alexander E Gorbalenya; Charles M Rice
Journal:  J Biol Chem       Date:  2004-08-31       Impact factor: 5.157

6.  Complete replication of hepatitis C virus in cell culture.

Authors:  Brett D Lindenbach; Matthew J Evans; Andrew J Syder; Benno Wölk; Timothy L Tellinghuisen; Christopher C Liu; Toshiaki Maruyama; Richard O Hynes; Dennis R Burton; Jane A McKeating; Charles M Rice
Journal:  Science       Date:  2005-06-09       Impact factor: 47.728

7.  Hepatitis C virus NS5A is a direct substrate of casein kinase I-alpha, a cellular kinase identified by inhibitor affinity chromatography using specific NS5A hyperphosphorylation inhibitors.

Authors:  Manuela Quintavalle; Sonia Sambucini; Vincenzo Summa; Laura Orsatti; Fabio Talamo; Raffaele De Francesco; Petra Neddermann
Journal:  J Biol Chem       Date:  2006-12-13       Impact factor: 5.157

8.  Mutational analysis of hepatitis C virus nonstructural protein 5A: potential role of differential phosphorylation in RNA replication and identification of a genetically flexible domain.

Authors:  Nicole Appel; Thomas Pietschmann; Ralf Bartenschlager
Journal:  J Virol       Date:  2005-03       Impact factor: 5.103

9.  Identification of residues required for RNA replication in domains II and III of the hepatitis C virus NS5A protein.

Authors:  Timothy L Tellinghuisen; Katie L Foss; Jason C Treadaway; Charles M Rice
Journal:  J Virol       Date:  2007-11-21       Impact factor: 5.103

10.  Domain III of NS5A contributes to both RNA replication and assembly of hepatitis C virus particles.

Authors:  Mair Hughes; Stephen Griffin; Mark Harris
Journal:  J Gen Virol       Date:  2009-03-04       Impact factor: 3.891
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  23 in total

1.  Expanding the proteome of an RNA virus by phosphorylation of an intrinsically disordered viral protein.

Authors:  Daniel G Cordek; Tayler J Croom-Perez; Jungwook Hwang; Michele R S Hargittai; Chennareddy V Subba-Reddy; Qingxia Han; Maria Fernanda Lodeiro; Gang Ning; Thomas S McCrory; Jamie J Arnold; Hasan Koc; Brett D Lindenbach; Scott A Showalter; Craig E Cameron
Journal:  J Biol Chem       Date:  2014-07-16       Impact factor: 5.157

Review 2.  Proteomic approaches to analyzing hepatitis C virus biology.

Authors:  Florian Douam; Alexander Ploss
Journal:  Proteomics       Date:  2015-05-05       Impact factor: 3.984

3.  Phosphorylated tyrosine 93 of hepatitis C virus nonstructural protein 5A is essential for interaction with host c-Src and efficient viral replication.

Authors:  Stefan Klinker; Sabine Stindt; Lothar Gremer; Johannes G Bode; Christoph G W Gertzen; Holger Gohlke; Oliver H Weiergräber; Silke Hoffmann; Dieter Willbold
Journal:  J Biol Chem       Date:  2019-03-12       Impact factor: 5.157

4.  Serine 235 Is the Primary NS5A Hyperphosphorylation Site Responsible for Hepatitis C Virus Replication.

Authors:  Shih-Chin Hsu; Chieh-Wen Lo; Ting-Chun Pan; Kuan-Ying Lee; Ming-Jiun Yu
Journal:  J Virol       Date:  2017-06-26       Impact factor: 5.103

5.  Nonstructural 5A Protein of Hepatitis C Virus Regulates Soluble Resistance-Related Calcium-Binding Protein Activity for Viral Propagation.

Authors:  Giao V Q Tran; Trang T D Luong; Eun-Mee Park; Jong-Wook Kim; Jae-Woong Choi; Chorong Park; Yun-Sook Lim; Soon B Hwang
Journal:  J Virol       Date:  2015-12-30       Impact factor: 5.103

6.  Y-Box Binding Protein 1 Stabilizes Hepatitis C Virus NS5A via Phosphorylation-Mediated Interaction with NS5A To Regulate Viral Propagation.

Authors:  Wei-Ting Wang; Tsung-Yuan Tsai; Chi-Hong Chao; Bo-Ying Lai; Yan-Hwa Wu Lee
Journal:  J Virol       Date:  2015-09-09       Impact factor: 5.103

7.  Hepatitis C Virus Particle Assembly Involves Phosphorylation of NS5A by the c-Abl Tyrosine Kinase.

Authors:  Shota Yamauchi; Kenji Takeuchi; Kazuyasu Chihara; Xuedong Sun; Chisato Honjoh; Hatsumi Yoshiki; Hak Hotta; Kiyonao Sada
Journal:  J Biol Chem       Date:  2015-07-22       Impact factor: 5.157

8.  Vinexin β Interacts with Hepatitis C Virus NS5A, Modulating Its Hyperphosphorylation To Regulate Viral Propagation.

Authors:  Wei Xiong; Jie Yang; Mingzhen Wang; Hailong Wang; Zhipeng Rao; Cheng Zhong; Xiu Xin; Lin Mo; Shujuan Yu; Chao Shen; Congyi Zheng
Journal:  J Virol       Date:  2015-05-13       Impact factor: 5.103

9.  Characterization of a Threonine-Rich Cluster in Hepatitis C Virus Nonstructural Protein 5A and Its Contribution to Hyperphosphorylation.

Authors:  Christian Schenk; Max Meyrath; Uwe Warnken; Martina Schnölzer; Walter Mier; Christian Harak; Volker Lohmann
Journal:  J Virol       Date:  2018-11-27       Impact factor: 5.103

10.  Phosphoproteomics Identified an NS5A Phosphorylation Site Involved in Hepatitis C Virus Replication.

Authors:  Weng Man Chong; Shih-Chin Hsu; Wei-Ting Kao; Chieh-Wen Lo; Kuan-Ying Lee; Jheng-Syuan Shao; Yi-Hung Chen; Justin Chang; Steve S-L Chen; Ming-Jiun Yu
Journal:  J Biol Chem       Date:  2015-12-23       Impact factor: 5.157
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