Clinicopathologic Features of IgA-Dominant Postinfectious Glomerulonephritis.

BACKGROUND: IgA-dominant acute postinfectious glomerulonephritis (APIGN) is a recently recognized morphologic variant of APIGN, but its clinicopathologic features were not clearly characterized. We will present demographic, clinical and renal biopsy findings from seven patients with IgA-dominant APIGN with a literature review.
METHODS: All renal biopsy specimens (n=1,119) processed by the Department of Pathology in Hanyang University Hospital from 2005 to 2009 were reviewed. Seven patients with IgA-dominant APIGN were identified, and their clinical data analyzed.
RESULTS: All patients had renal failure, hematuria and proteinuria. One was diabetic, and none of the patients had previous renal diseases. Three had clinical infections at the time of presentation: 2 with methicillin-resistant Staphylococcus aureus and one with rickettsial infection. Light microscopically diffuse endocapillary proliferative and exudative glomerulonephritis was found in all cases. Immunofluorescence microscopy showed granular IgA deposits along peripheral capillary walls and in mesangium. Ultrastructurally, subepithelial 'humps' with mesangial deposits were noted. End-stage renal disease developed in two patients, chronic renal failure was stationary in two, and azotemia improved in three.
CONCLUSIONS: Various infections including rickettsiosis preceded IgA-dominant APIGN in both diabetics and nondiabetics. Because the prognosis of IgA-dominant APIGN is poor, early diagnosis based on renal biopsy is required.

Acute postinfectious glomeruonephritis (APIGN) is an immune complex-mediated form of glomerulonephritis that follows non-renal infection, often caused by Streptococci. It mostly affects children after an upper respiratory tract infection or impetigo. The typical histological pattern of APIGN includes: diffuse endocapillary proliferative and exudative glomerulonephritis (GN) on light microscopy (LM), coarsely granular deposition of IgG and C3 in a predominantly capillary wall distribution on immunofluorescence (IF), and characteristic subepithelial hump-shaped deposits on electron microscopy (EM).

Over recent decades, the spectrum of the disease has been changed. Not only Streptococci but also other bacterial, viral, and parasitic organisms have been implicated in the pathogenesis of postinfectious glomerulonephritis (PIGN).1-3 Extensive use of renal biopsy has demonstrated the presence of atypical histological features of PIGN. Recently, there have been several reports of PIGN with IgA-dominant immune complex deposition, mostly related to staphylococcal infection, especially in patients with underlying diabetic nephropathy.4-19 IgA-dominant PIGN is characterized by diffuse endocapillary proliferative GN on LM, intense deposits of IgA as the dominant or co-dominant immunoglobulin on IF, along with mesangial and subepithelial electron dense deposits on EM.9 However, there have been additional reports of IgA-dominant PIGN that lacked typical features such as diffuse endocapillary hypercellularity, subepithelial deposits, underlying diabetes mellitus and history of infection.11,12,15,17-19 Therefore, to avoid potential ambiguity, the clinicopathological features of IgA-dominant PIGN need to be investigated.

Here we present seven cases of APIGN which showed IgA-dominant deposition on renal biopsy. Clinical features and renal pathologic findings were analyzed and discussed with a review of the literature.

MATERIALS AND METHODS

All renal biopsies (n=1,119) processed by the Department of Pathology, Hanyang University Hospital, Seoul, Korea from January 2005 to December 2009 were reviewed retrospectively, and fifteen patients with diagnosis of APIGN were identified. The diagnosis of APIGN was made based on the presence of at least three of the known five criteria.14 We excluded patients with systemic lupus erythematosus, Henoch-Schönlein purpura or IgA nephropathy.15 Among 15 cases diagnosed as APIGN, seven cases showed glomerular dominant or codominant IgA deposits. The medical records of the patients were reviewed for age, gender, underlying diseases, type and cause of infection, clinical presentation, laboratory data including baseline and peak serum creatinine, serum C3 and C4 level, 24-hour urine protein, and urine analysis. We also investigated the modalities of treatment and clinical outcomes. We defined acute renal failure (ARF) as an increase of serum creatinine of at least 0.5 mg/dL above baseline values, and remission of ARF as the normalization of serum creatinine to baseline levels. End-stage renal disease (ESRD) was defined as requiring maintenance dialysis therapy.

All 7 renal biopsy samples were processed according to standard techniques of LM, IF, and EM. For each patient, 6 slides which were stained with hematoxylin and eosin, Masson's trichrome, periodic acid-Schiff and Jones methenamine silver were reviewed. Systematic analysis on the morphologic changes of glomeruli, tubules, interstitium, and vessels was done according to the practical standardization in renal biopsy reporting.20 IF staining had been performed on 3-µm cryostat sections using polyclonal fluorescein-isothiocyanate-conjugated antibodies to IgG, IgM, IgA, C3, C1q, C4, kappa, lambda, fibrinogen and albumin (DakoCytomation, Glostrup, Denmark). The intensity of IF staining was graded on a scale of 0 to 3+. Samples were prepared for ultrastructural evaluation by standard techniques and examined under a Hitachi H-7600S electron microscope (Hitachi Ltd., Tokyo, Japan) at 80 kV.

RESULTS

Clinical features

The clinical features and demographic data of the patients diagnosed as having IgA-dominant PIGN are shown in Table 1. The patients consisted of 6 males and 1 female, with a mean age of 65 years (range, 31 to 84 years). All seven patients presented with ARF, proteinuria and hematuria. The time interval between onset of clinical infection and ARF was 2 to 5 weeks. Basal mean serum creatinine level was 1.0 mg/dL (range, 0.7 to 1.2 mg/dL) and this increased to 3.1 mg/dL (range, 1.9 to 4.7 mg/dL). Hypocomplementemia was present in 2 (patients 3 and 5) out of 7 patients. IgA levels were elevated in patient 4 (551 mg/dL; normal range, 82 to 453 mg/dL) and patient 7 (811 mg/dL). Patient 2 and 3 had hypertension, patient 4 alcoholic liver cirrhosis, and patient 5 diabetes mellitus without nephropathy. None of the patients had underlying renal diseases.

Table 1

IgA-dominant acute postinfectious glomerulonephritis: demographic and clinical features

M, male; F, female; HTN, hypertension; DM, diabetes mellitus; MRSA, methicillin-resistant Staphylococcus aureus; GN, glomerulonephritis; Cr, serum creatinine; UA, routine urinalysis; WBC, white blood cell; HPF, high power field; RBC, red blood cell; ESRD, end-stage renal disease.

Clinical infections were documented in three patients at the time of biopsy. Two patients (patient 1 and patient 7) had methicillin-resistant Staphylococcus aureus (MRSA) infections, and one patient (patient 5) had a rickettsial infection. The MRSA infections were infectious arthritis of the knee joint in patient 1 and pneumonia in patient 7. In the remaining 4 patients (patients 2, 3, 4, and 6), no pathogens had been detected.

Pathology findings

Light microscopy

Renal biopsy findings are summarized in Tables 2 and 3. All seven patients showed acute diffuse proliferative GN with diffuse endocapillary proliferation and prominent intracapillary infiltration of neutrophils and some mononucleated cells (Fig. 1). Small or circumferential cellular crescents associated with segmental fibrinoid necrosis were present in five patients (range, 9 to 67%, affected >50% of glomeruli in one patient), and there was evidence of fibrin thrombi in four cases. Varying degrees of global glomerulosclerosis (6-50%) were noted in four cases. None of the patients had features associated with diabetic nephropathy.

Table 2

IgA-dominant acute postinfectious glomerulonephritis: light microscopic findings in renal biopsy

Lesions are graded from 0 to 3+ (0, absent; 1+, mild; 2+, moderate; 3+, marked).

GS, global sclerosis; SS, segmental sclerosis; EC, endocapillary; MES, mesangial.

aTubulointerstitial nephritis associated with acute tubular necrosis.

Table 3

IgA-dominant acute postinfectious glomerulonephritis: immunofluorescence and electron microscopic findings in renal biopsy

Deposits are graded from 0 to 3+ (0, absent; 1+, mild; 2+, moderate; 3+, marked).

IF, immunofluorescence; MES, mesangial; GCW, glomerular capillary wall; EM, electron microscopy.

aNo glomerulus included in EM sample.

Fig. 1

Light microscopy findings in renal biopsy (patient 1). Most glomeruli show diffuse endocapillary hypercellularity and neutrophilic infiltration with cellular crescents. Tubulointerstitial changes are mild (periodic acid-Schiff [PAS]) (A). In high-power views, the glomerulus shows a cellular crescent as well as endocapillary hypercellularity (PAS) (B), diffuse mesangial and endocapillary hypercellularity (methenamine silver) (C), and subepithelial, 'hump'-like deposits (arrow) (Masson's trichrome) (D).

All seven patients had some degree of interstitial inflammation, consisting predominantly of lymphocytes. Tubular atrophy and interstitial fibrosis were mild in six patients and moderate in the remaining patient (patient 6). Patient 6 also exhibited marked tubulointerstitial inflammation and granular casts, corresponding features associated with acute tubular injury. There was some degree of vascular change in most of the patients. Arterial intimal thickening was mild in five of the patients and moderate in one (patient 5), while mild arteriolosclerosis was noted in four patients (Table 2).

IF microscopy

The IF samples contained about 3 glomeruli (range, 1 to 8). IgA was the dominant class of immunoglobulin deposited in the glomeruli (intensity, 1+ to 3+) (Table 3), and there was a coarsely granular pattern of glomerular capillary wall and/or mesangial staining for IgA and C3 (Fig. 2). Most patients showed no IF staining for C1q and C4, but displayed variable intensities of mesangial and/or glomerular capillary wall staining for IgG. In five patients there was trace to weak IgM staining in the mesangium. In six patients staining for the κ and λ light chains was approximately the same (Table 3).

Fig. 2

Representative photomicrographs of immunofluorescence (IF) staining (patient 2). IF staining for IgG (A) is negative, but there is conspicuous coarsely granular positive IF staining for IgA (B) and C3 (C) along the glomerular capillary wall (3+), and segmentally in the mesangial areas (2+). IF staining for kappa (D) is weakly positive, and lambda (E) shows 1+ segmentally staining in mesangial areas and peripheral capillary walls.

Electron microscopy

Ultrastructural analysis was performed in all but one case (patient 1) in which the EM specimen contained no glomerulus. They had varying numbers of typical subepithelial 'humps' and/or intramembranous deposits as well as some mesangial electron dense deposits (Fig. 3). Some subepithelial deposits showed partial or extensive resorption with partial loss of electron density (Fig. 3C). Endocapillary hypercellularity and neutrophilic infiltration were prominent. Foot process effacement was mostly diffuse, estimating about 90% of the external capillary surface except for one (patient 6, 40%) (Table 3).

Fig. 3

Electron microscopic features of two renal biopsies (patients 3 and 5). (A, B) Patient 3 show subepithelial electron dense deposits ('humps'), intramembranous and mesangial deposits. (C) Patient 5 shows a large subepithelial deposit with proliferation of endothelial and mesangial cells, and a neutrophil in the lumen. Partially resorbed subepithelial 'hump' with electron lucency. In (D), there is a broad based subepithelial deposit (arrows) attracting neutrophil in the capillary lumen (A, B, ×8,000; C, ×7,000; inset in C, ×15,000; D, ×12,000).

Treatment and follow-up

Three patients (patients 1, 5, and 7) were treated with antibiotics because they had had definite episodes of infection. Patient 1 progressed to ESRD and had maintenance hemodialysis, while the renal failure in patient 5 improved in response to antibiotic therapy. Patient 7 did not respond to the antibiotics, but his renal function was improved after treatment with high-dose prednisolone. In contrast, patient 3, who was treated with corticosteroid and cyclophosphamide, showed no improvement in renal function and died of complications including necrotizing pneumonia and bacterial meningitis. The remaining three patients (patients 2, 4, and 6), who had had no definite episodes of infection, were conservatively treated. The renal failure in patients 2 and 4 was stable and stationary, whereas azotemia in patient 6 resolved itself.

DISCUSSION

Recently, a unique form of PIGN characterized by IgA-dominant deposits has been increasingly recognized.5-19 At this time of writing, eight-five cases have been reported in the English literatures.5-19 Their clinical and pathological findings are summarized in Table 4.

Table 4

Review of literatures: previously reported 85 cases of IgA-dominant acute postinfectious glomerulonephritis

M, male; MesPGN, mesangial proliferative glomerulonephritis; F, female; MRSA, methicillin-resistant Staphylococcus aureus; NS, nephrotic syndrome; GN, glomerulonephritis; RPGN, rapidly progressive glomerulonephritis; DPGN, diffuse proliferative (mesangial and endocapillary) glomerulonephritis; HD, hemodialysis; MSSA, methicillin-sensitive Staphylococcus aureus; CKD, chronic kidney disease; HTN, hypertension; ARF, acute renal failure; CRF, chronic renal failure; PGN, endocapillary proliferative glomerulonephritis; MRSE, methicillin-resistant Staphylococcus epidermidis; HIV, human immunodeficiency virus; KT, kidney transplantation; MSSE, methicillin-sensitive Staphylococcus epidermidis; IVD, iv drug use; CMRSA, community-associated methicillin-resistant Staphylococcus aureus-10; COPD, chronic obstructive pulmonary disease.

Here we presented seven patients with biopsy-proven IgA-dominant APIGN along with clinical and pathological findings. Four of them were over 60 years of age, and the average age was 65 years. In other reports, the average age at diagnosis was 60 years (reported range, 16 to 89 years), and 61% of the reported patients were 65 years or older.5-19 Thus, the elderly should be predisposed to IgA-dominant PIGN.19 Our patients also had a male predominance of 6:1, similar to other reports.5-19

In our study, only one patient was diabetic and another had alcoholic liver cirrhosis. Previous studies reported that IgA-dominant APIGN was mainly associated with diabetes mellitus.9,12,14,15,17-19 Thus, diabetes was known to be the important risk factor for IgA-dominant APIGN. However, we showed that many non-diabetic patients could be exposed to IgA-dominant APIGN.

The infectious agent was identified in 3 patients (43%) within the study, and Staphylococcus aureus was the most common (67%) cause of infection. It took from 2 to 5 weeks between the clinical onset of infection and the renal symptoms. In other studies, the average duration of the incubation period was estimated to be about 4 weeks.5-19 In some cases, the infection may be identified at the time of renal biopsy because infection was unrecognized for some time.21

All of the patients had renal insufficiency, hematuria, and proteinuria at presentation. More than a half of them showed advanced azotemia (serum creatinine >3 mg/dL). In other reports, the peak serum creatinine ranged from 1.2 to 14.5 mg/dL (mean, 4.0 mg/dL).5-19 Also, all patients were nephrotic, having a mean 24-hour urine protein of 5.2 g. In contrast, previous studies reported 44% of patients having had nephrotic syndrome although proteinuria is a universal presenting feature (reported range, 0.15 to 15 g/day).5-19 Hypocomplementemia may be an important clue to diagnose PIGN. However, it was present in two patients (29%) in this study. Complement levels were not consistently depressed in other studies as well.5-8,10-12,15-19

Antibiotic therapy is considered mainstay for IgA-dominant APIGN following episodes of infection,8 but it is not always successful. In this study, two of three patients who were treated with antibiotics did not respond to the therapy. One of them showed improvement in renal function after steroid therapy. Steroid treatment is a possible alternative for IgA-dominant APIGN presenting renal insufficiency when the renal function fails to improve after antibiotic therapy.8,11,15,18

In this study, three patients (42%) had complete recovery of renal function with normalization of serum creatinine and decreasing proteinuria. Two patients (29%) had partial recovery with moderate reduction of proteinuria and serum creatinine. Two patients (29%) remained dialysis-dependent and one patient (14%) died. Before the 1990s, complete remission of PIGN was reported in 60-80% of the patients.21 According to the recent studies, however, it was reported in 43%.19 The prognosis of IgA-dominant PIGN is less favorable than that of typical PIGN.19-22

Previous studies have shown that endocapillary proliferative and exudative GN is the predominant histological pattern of IgA-dominant PIGN (Table 4). Pure mesangial proliferative and crescentic GN were described in 34% and 6% of cases, respectively.5-19 All our patients had diffuse endocapillary proliferation, and five patients (71%) had small or circumferential cellular crescents with fibrinoid necrosis. On IF, IgA was the dominant immunoglobulin in all our patients, with or without weaker staining for IgG. There was high-intensity staining for C3 as well. This finding is a considerable contrast to IgA nephropathy, in which C3 staining is typically weaker than IgA staining.22 Ultrastructurally, mesangial electron dense deposits were seen in all our cases (100%), subepithelial deposits in five (83%), intramembranous in five (83%), and subendothelial in two (33%). One patient showed extensive resorption of subepithelial deposits. Subepithelial deposits, frequently exhibiting a hump-shaped appearance, were common. Subendothelial deposits were less frequent and small, consistent with other reports.5-19

In this study, we documented for the first time an association between rickettsial infection and IgA-dominant APIGN. Rickettsial infection is considered as a possible cause of IgA-dominant APIGN, especially in areas where high incidences of rickettsial disease is apparent. The histological features of rickettsial infection generally include interstitial nephritis, vasculitis, acute tubular necrosis and occasionally, APIGN.23 Various humoral immune responses have been suggested to be associated with increased serum IgA levels or IgA-containing circulating immune complexes in rickettsial infection.24,25 In response to the rickettsial infection, interleukin (IL)-6 and IL-10 levels may be increased to stimulate plasma cell growth and differentiation.24,25

The mechanism of selective IgA deposition in PIGN is unclear. It may be related to increased serum levels of IgA and IgA-containing circulating immune complexes, possibly as a result of subclinical mucosal infection or decreased IgA hepatic clearance caused by serum IgA1 hypersialylation.14,26 A role of superantigens has been suggested.6,7 Superantigens are proteins that may be detected in several pathogenic microbes including Staphylococcus. They are powerful activators of immune systems and stimulate the production of T cells along with a variety of cytokines including tumor necrosis factor-α. They also induce antibody production including IgA. Staphylococcal enterotoxins such as enterotoxin B and C can act as superantigens and have been identified in staphylococcal-associated GN.6,7 Interestingly, IgA-dominant PIGN may also develop following coagulase-negative Staphylococcus which does not produce enterotoxin, suggesting the potential pathogenic role for particular staphylococcal cell surface antigens.22 Regarding nonstaphylococcal pathogens, Endo et al.27 demonstrated that various strains of gram-negative bacteria including Pseudomonas aeruginosa, Escherichia coli, Hemophilus influenza, and Klebsiella pneumoniae, can induce glomerular deposition of IgA and C3 in animal studies. Many patients with IgA-dominant APIGN are diabetic.9,12,14,15,17-19 It is possible that diabetes plays a role in the provocation of an IgA-dominant immune response. Many studies have shown that diabetes have high levels of serum IgA and IgA-containing circulating immune complexes compared with non-diabetes.22 Proposed explanation for the high serum IgA levels in diabetes include decreased IgA hepatic clearance caused by serum IgA1 hypersialylation and increased synthesis of IgA in association with subclinical mucosal infection or as a potential immune response to advanced glycation endproduct.22

IgA-dominant APIGN may be difficult to differentiate from IgA nephropathy. In several studies, IgA-dominant APIGN can be diagnosed with a high degree of certainty when having ARF at presentation, diffuse endocapillary proliferation with neutrophil infiltration, glomerular capillary wall, and/or when mesangial staining on IF and subepithelial humps are present.5-19,22 However when resolving lesions are present with mesangial proliferation associated with mesangial patterns of immune deposits and loss of subepithelial humps, it is difficult to distinguish IgA-dominant APIGN from IgA nephropathy. In our study, patient 6 had no history of infection and no subepithelial humps on EM. However she was diagnosed as IgA-dominant APIGN rather than IgA nephropathy. Her renal biopsy showed a typical finding of APIGN, particularly in early resolving APIGN that there were IgA and C3 deposits along glomerular capillary wall and mesangium, and localized electron dense deposits on notch regions overlying mesangial areas. The histological findings largely depend on the timing of renal biopsies. The acute phase of APIGN may be subclinical, and detected only on renal biopsy performed enthusiastically because of asymptomatic renal failure or microscopic urinary abnormalities.

In summary, extensive use of renal biopsy has demonstrated the presence of an atypical feature of APIGN, glomerular IgA-dominant deposition. It is most common in the elderly and occurs in both diabetics and non-diabetics. It typically occurs in association with staphylococcal infections, and presents with renal failure, hematuria, and proteinuria. Hypocomplementemia may be an important clue to the diagnosis of PIGN, but inconsistently present. Antibiotic therapy is considered mainstay, and steroid therapy may be a possible alternative when IgA-dominant APIGN is accompanied by renal insufficiency and does not respond to antibiotic treatments. Prognosis is less favorable than typical APIGN. Histologically, most cases exhibit endocapillary hypercellularity and neutrophil infiltration. In renal biopsies, IgA is the dominant or co-dominant immunoglobulin deposited in the glomeruli. Rickettsial infection can cause IgA-dominant APIGN and should be considered as a possible cause of APIGN in areas where high incidence of rickettsial disease occur. Greater awareness of the presentation and course of IgA-dominant APIGN is necessary to ensure prompt diagnosis and treatment.